Marker removal strategies were developed for Thermoanaerobacterium saccharolyticum to select against the pyrF gene and the pta and ack genes. The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δldh Δpta Δack strain that produces ethanol at a high yield.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3067457 | PMC |
| http://dx.doi.org/10.1128/AEM.01731-10 | DOI Listing |
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