AI Article Synopsis
- The study examines the link between telomerase activity and the expression of its components (hTR and hTERT) in lung cancer tissues compared to adjacent non-cancerous tissues.
- In 68 lung cancer samples, telomerase activity was found in 79.4% of cases, while hTR and hTERT showed high expression rates (98.5% and 91.2% respectively), contrastingly, hTERT was rarely found in normal tissues (10.3%).
- Results indicate that hTERT expression is closely tied to telomerase activity, suggesting that telomerase potentially contributes to lung cancer development and might be regulated at the
Article Abstract
Background: To investigate the relationship between the telomerase activity and telomerase component expression in lung cancer, and to explore whether telomerase activity is regulated at the gene or transcriptional level.
Methods: Expression of the hTR and hTERT were detected in 68 human lung cancer tissues and responsive adjacent non-neoplastic lung tissues by semi-quantitative reverse transcription-polymerase chain reaction. Telomerase activity was detected by telomeric repeat amplification protocol.
Results: In 68 lung cancer tissues, the positive rate of the telomerase activity, hTR and hTERT expression were 79.4% (54/68), 98.5% (67/68) and 91.2% (62/68) respectively. Whereas most adjacent non-neoplastic lung tissues expressed hTR (62/68, 91.2%) also, hTERT was detected in only 7 (10.3%) normal lung tissues and no telomerase activity were detected in the 68 non-neoplastic lung cancer tissues. As compared with hTR, hTERT expression was closely related to telomerase activity. The concordance was 89.0% (121/136), whereas the concordance between telomerase and hTR was 43.4% (59/136).
Conclusions: The results suggest that telomerase may play an important role in tumorigenesis of lung cancer. Telomerase activity may be regulated in transcription level or translation level.
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| http://dx.doi.org/10.3779/j.issn.1009-3419.2002.01.09 | DOI Listing |
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