AI Article Synopsis
- The mechanisms governing embryonic stem cell (ESC) pluripotency are not fully understood, with prior research linking the Janus kinase-STAT3 pathway and ERK/GSK3 signaling to mESC maintenance.
- New findings show that inhibiting protein kinase C (PKC) isoforms can also preserve mESC pluripotency without activating STAT3 or inhibiting ERK/GSK3 pathways.
- Specifically, the atypical PKC isoform, PKCζ, promotes lineage commitment in mESCs, indicating that PKC signaling is essential for balancing ESC self-renewal and differentiation.
Article Abstract
The intricate molecular mechanisms that regulate ESC pluripotency are incompletely understood. Prior research indicated that activation of the Janus kinase-signal transducer and activator of transcription (STAT3) pathway or inhibition of extracellular signal-regulated kinase/glycogen synthase kinase 3 (ERK/GSK3) signaling maintains mouse ESC (mESC) pluripotency. Here, we demonstrate that inhibition of protein kinase C (PKC) isoforms maintains mESC pluripotency without the activation of STAT3 or inhibition of ERK/GSK3 signaling pathways. Our analyses revealed that the atypical PKC isoform, PKCζ plays an important role in inducing lineage commitment in mESCs through a PKCζ-nuclear factor kappa-light-chain-enhancer of activated B cells signaling axis. Furthermore, inhibition of PKC isoforms permits derivation of germline-competent ESCs from mouse blastocysts and also facilitates reprogramming of mouse embryonic fibroblasts toward induced pluripotent stem cells. Our results indicate that PKC signaling is critical to balancing ESC self-renewal and lineage commitment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413330 | PMC |
| http://dx.doi.org/10.1002/stem.605 | DOI Listing |
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