Stromelysin-1 (matrix metalloproteinase-3: MMP-3) occupies a central position in collagenolytic and elastolytic cascades, leading to cutaneous intrinsic and extrinsic aging. We screened extracts of a propolis sample from Algeria with the aim to isolate compounds able to selectively inhibit this enzyme. A butanolic extract (B (3)) of the investigated propolis sample was found to potently inhibit MMP-3 activity (IC (50) = 0.15 ± 0.03 µg/mL), with no or only weak activity on other MMPs. This fraction also inhibited plasmin amidolytic activity (IC (50) = 0.05 µg/mL) and impeded plasmin-mediated proMMP-3 activation. B (3) was fractionated by HPLC, and one compound, characterized by NMR and mass spectroscopy and not previously identified in propolis, i.e., (+)-chicoric acid, displayed potent IN VITRO MMP-3 inhibitory activity (IC (50) = 6.3 × 10 (-7) M). In addition, both caffeic acid and (+)-chicoric acid methyl ester present in fraction B (3) significantly inhibited UVA-mediated MMP-3 upregulation by fibroblasts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1055/s-0030-1270713 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Physicochemical, Antioxidant Characteristics and Sensory Evaluation of Functional Pro-Biogenic Ice Cream.
Food Sci Nutr
January 2025
Department of Food Science and Technology, Faculty of Pharmacy, Tehran Medical Science Islamic Azad University Tehran Iran.
Pro-biogenic is a recent terminology widely used for products that combine biogenic materials and probiotics which has made progressive improvement in a new era of research on functional foods. This study aimed to develop functional ice cream with and propolis extract (PE) as a biogenic part to develop ice cream's physiochemical and antioxidant characteristics. Five probiotic ice cream samples were prepared using different levels of PE powder (0%, 0.
View Article and Find Full Text PDFEfficacy of non -thermal pressure plasma versus other modalities for disinfection of primary root canals.
BMC Oral Health
January 2025
Associate Professor of Operative Dentistry, Conservative Dentistry Department, Faculty of Oral and Dental Medicine Badr University in Cairo, Cairo, Egypt.
Background: Endodontic treatment aims in the preservation of extremely carious primary teeth. For root canal therapy to be successful, root canals must be properly prepared and effectively irrigated .Therefore, it is necessary to select the proper root canal disinfection method to preserve the primary tooth.
View Article and Find Full Text PDFDetection of glyphosate, glufosinate, and their metabolites in multi-floral honey for food safety.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
Department of Veterinary Medicine and Animal Science (DIVAS), University of Milan, Lodi, Italy.
Beehives can accumulate environmental contaminants as bees gather pollen, propolis, and water from their surroundings, contaminating hive products like honey. Moreover, in multifloral environments, bees can interact with plants treated with different pesticides, often causing higher pesticides concentrations in multi-floral honey than in mono-floral varieties. Glyphosate and glufosinate are both widely used herbicides.
View Article and Find Full Text PDFEnzyme Inhibitory, Physicochemical, and Phytochemical Properties and Botanical Sources of Honey, Bee Pollen, Bee Bread, and Propolis Obtained from the Same Apiary.
Antioxidants (Basel)
December 2024
Department of Chemistry and Biochemistry, Faculty of Agriculture, University of Belgrade, 11080 Belgrade, Serbia.
Bee products are an important source of nutrients and bioactive phytochemicals. This study aimed to determine the chemical composition (proximate composition, general phytochemical composition, sugar, and phenolic profiles) of four different products (honey, bee pollen, bee bread, and propolis), obtained from the same apiary, as well as to assess their biological activity through antioxidant and enzyme inhibition assays (α-amylase, α-glucosidase, lipase, AchE, neuraminidase, angiotensin-converting enzyme, urease, trypsin, tyrosinase, carbonic anhydrase, thioredoxin reductase, adenosine deaminase). Clear differences were observed among the samples in terms of both chemical composition and biological activity.
View Article and Find Full Text PDFWhite adipose tissues and skeletal muscles as a target of chrysin during the treatment of obesity in rats.
Sci Rep
January 2025
Department of Biochemistry, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, EL-Hadara, POB 21561, Alexandria, Egypt.
Obesity is a rapidly growing epidemic that continues to be a major severe health problem due to its association with various adverse health consequences. Since 1975, the WHO estimates that the prevalence of obesity has tripled globally. Chrysin is a flavone that is mostly found in the Passiflore species of plants and in propolis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!