Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity.

Chem Commun (Camb)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong RD 130, Shanghai, 200237, China.

Published: February 2011

We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

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Source
http://dx.doi.org/10.1039/c0cc04887aDOI Listing

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