AI Article Synopsis
- Researchers developed various polymers from MPC to test their ability to mimic cell membranes and capture target proteins.
- Hydrophilic MPC-based polymers reduced non-specific protein binding and effectively captured carbonic anhydrase II when tested with Sul.
- However, all polymers with PC groups failed to capture Cyclooxygenase-1 when using ibuprofen as a probe, indicating that the placement of PC groups influenced the polymer's interactions with proteins.
Article Abstract
We have prepared several types of polymers derived from 2-methacryloyloxyethyl phosphorylcholine (MPC) to evaluate whether polymers of MPC work as cell membrane mimic or not. We firstly applied capturing test of target proteins of 4-carboxybenzenesulfonamide (Sul) or ibuprofen (Ibu) as a probe. As the results, the rather hydrophilic polymers based on MPC were able to suppress non-specific binding proteins as expected. Additionally, some of the MPC based polymeric surface was able to capture greater amount of carbonic anhydrase II than those of other polymers, when Sul was utilized as probe. In contrary, all the polymers having PC groups and Ibu probe were unable to capture Cyclooxygenase-1 (COX-1), its target protein. These results suggested that the position of PC groups realized hydrophilic polymer surface, while MPC based polymer was not able to supply the suitable environment for COX-1 to interact with Ibu.
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| http://dx.doi.org/10.1016/j.colsurfb.2010.12.033 | DOI Listing |
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