Novel nested direct PCR technique for malaria diagnosis using filter paper samples.

J Clin Microbiol

Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria.

Published: April 2011

The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122810PMC
http://dx.doi.org/10.1128/JCM.01792-10DOI Listing

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