The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122810 | PMC |
http://dx.doi.org/10.1128/JCM.01792-10 | DOI Listing |
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