Objective: To investigate the biological effects of ultraviolet B (UVB) irradiation on human bronchial epithelial cells (16HBE cells) and explore the possible mechanism.
Methods: The survival rates of 16HBE cells were detected by MTT assay at 12 h after UVB irradiation at different doses (0, 10, 30, 50, 70, and 100 J/m(2)) or at 50 J/m(2) for different durations (2, 4, 8, 12, and 24 h). The DNA ladder was detected by agarose gel electrophoresis, the cell cycle changes were analyzed by flow cytometry, and the expression of nuclear factor-κB (NF-κB)/p65 protein was assayed by Western blotting following the exposures.
Results: UVB irradiation of the cells resulted in lowered cell survival rates, DNA fragmentation, S phase arrest and up-regulation of NF-κB/p65 protein expression.
Conclusions: UVB irradiation can induce growth inhibition and apoptosis of 16HBE cells, in which process NF-κB protein may play a key role.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
UV-B induced flavonoid accumulation and related gene expression in blue- grained wheat at different periods of time.
Front Plant Sci
December 2024
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
Introduction: UV-B can be used as an additional technique for nutrient accumulation in blue-grained wheat, which has special nutritional properties due to its blue starch layer. The concentration of flavonoids in blue-grained wheat under UV-B irradiation is extremely important for further investigation and exploitation of the nutritional properties of blue-grained wheat.
Methods: This investigation focuses on the expression of flavonoids and associated genes in blue-grained wheat using transcriptomic and metabolomic analyzes.
Enhanced Synthesis of Volatile Compounds by UV-B Irradiation in Leaves.
Metabolites
December 2024
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Volatile compounds have a deep influence on the quality and application of the medicinal herb ; however, little is known about the effect of UV-B radiation on volatile metabolites. We herein investigated the effects of UV-B exposure on the volatile compounds and transcriptome of to assess the potential for improving its quality and medicinal characteristics. Out of 733 volatiles obtained, a total of 133 differentially expressed metabolites (DEMs) were identified by metabolome analysis.
View Article and Find Full Text PDFUltraviolet-B irradiation expands skin-resident CD81Foxp3 regulatory T cells with a highly activated phenotype.
J Invest Dermatol
December 2024
Department of Immunology, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan. Electronic address:
Exposure to ultraviolet-B (UVB) induces the expansion of regulatory T (Treg) cells expressing proenkephalin (PENK) and amphiregulin (AREG) with a healing function in the skin. It is unclear how this UVB exposure affects the functionally distinct subsets of skin Treg cells. In this study, we have demonstrated that skin-resident CD81Treg cells expressing both Penk and Areg expanded after UVB irradiation.
View Article and Find Full Text PDFCombination Therapy with Baricitinib and Narrowband Ultraviolet B for Active Non-Segmental Vitiligo: A Retrospective Controlled Study.
Clin Cosmet Investig Dermatol
December 2024
Department of Dermatology, Air Force Medical Center, PLA, Beijing, People's Republic of China.
Background: Vitiligo is a chronic autoimmune disease manifested by depigmented patches of skin devoid of melanocytes. Baricitinib, a JAK inhibitor selectively targeting JAK1/2, has shown preliminary efficacy for vitiligo. We aimed to assess the efficacy and tolerability of combination therapy with baricitinib and narrowband UV-B (NB-UVB) to treat active nonsegmental vitiligo (NSV).
View Article and Find Full Text PDFUrsolic Acid as a Protective Agent Against UVB-Induced Metabolic and Epigenetic Alterations in Human Skin Keratinocytes: An Omics-Based Study.
Cancer Prev Res (Phila)
December 2024
Rutgers, The State University of New Jersey, Piscataway, NJ, United States.
This study aimed to assess how ursolic acid (UA) can protect human skin keratinocytes from damage caused by ultraviolet B (UVB) radiation. Utilizing an omics-based approach, we characterized the features of photodamage and investigated the potential of UA to reverse HaCaT cell subpopulation injury caused by UVB radiation. The most significant changes in metabolite levels after UA treatment were in pathways associated with phosphatidylcholine biosynthesis, arginine and proline metabolism.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!