UV light below 300 nm is shown to generate the first photocycle intermediate in the blue light photoreceptor Photoactive Yellow Protein. Fluorescence and ultrafast transient absorption measurements indicate two excitation pathways: UV-B absorption by the chromophore and Fluorescence Resonant Energy Transfer (FRET) from tryptophan and tyrosine residues.
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http://dx.doi.org/10.1039/c0pp00274g | DOI Listing |
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