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Temperature-pressure phase diagram of a heterogeneous anionic model biomembrane system: results from a combined calorimetry, spectroscopy and microscopy study. | LitMetric

AI Article Synopsis

  • Researchers used advanced techniques like FT-IR spectroscopy, DSC, PPC, and others to analyze the phase behavior of a complex lipid mixture, including cholesterol, under varying temperature and pressure conditions.
  • The study covered temperatures from 5 to 65 °C and pressures up to 16 kbar to create a detailed phase diagram for this model lipid membrane.
  • The findings indicated that even with the addition of anionic lipids, the mixture maintained similar phase behavior to a neutral model, providing insights into protein interactions with lipid bilayers in challenging environmental conditions.

Article Abstract

By using Fourier transform infrared (FT-IR) spectroscopy in combination with differential scanning calorimetry (DSC) coupled with pressure perturbation calorimetry (PPC), ultrasound velocimetry, Laurdan fluorescence spectroscopy, fluorescence microscopy and atomic force microscopy (AFM), the temperature and pressure dependent phase behavior of the five-component anionic model raft lipid mixture DOPC/DOPG/DPPC/DPPG/cholesterol (20:5:45:5:25 mol%) was investigated. A temperature range from 5 to 65 °C and a pressure range up to 16 kbar were covered to establish the temperature-pressure phase diagram of this heterogeneous model biomembrane system. Incorporation of 10-20 mol% PG still leads to liquid-ordered (l(o))-liquid-disordered (l(d)) phase coexistence regions over a wide range of temperatures and pressures. Compared to the corresponding neutral model raft mixture (DOPC/DPPC/Chol 25:50:25 mol%), the p,T-phase diagram is - as expected and in accordance with the Gibbs phase rule - more complex, the phase sequence as a function of temperature and pressure is largely similar, however. This anionic heterogeneous model membrane system will serve as a more realistic model biomembrane system to study protein interactions with anionic lipid bilayers displaying liquid-disordered/liquid-ordered domain coexistence over a wide range of the temperature-pressure plane, thus allowing also studies of biologically relevant systems encountered under extreme environmental conditions.

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Source
http://dx.doi.org/10.1016/j.bbamem.2011.01.011DOI Listing

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