AI Article Synopsis
- Coxiella burnetii is the bacteria responsible for Q fever, and the Netherlands is experiencing its largest outbreak with nearly 4,000 human cases.
- A new single-nucleotide-polymorphism (SNP) genotyping method was developed to analyze clinical samples and distinguish between different C. burnetii strains.
- The study found that the Dutch outbreak is linked to at least 5 different genotypes, with some strains also present in livestock on multiple farms.
Article Abstract
Coxiella burnetii is the etiological agent of Q fever. Currently, the Netherlands is facing the largest Q fever epidemic ever, with almost 4,000 notified human cases. Although the presence of a hypervirulent strain is hypothesized, epidemiological evidence, such as the animal reservoir(s) and genotype of the C. burnetii strain(s) involved, is still lacking. We developed a single-nucleotide-polymorphism (SNP) genotyping assay directly applicable to clinical samples. Ten discriminatory SNPs were carefully selected and detected by real-time PCR. SNP genotyping appeared to be highly suitable for discrimination of C. burnetii strains and easy to perform with clinical samples. With this new method, we show that the Dutch outbreak is caused by at least 5 different C. burnetii genotypes. SNP typing of 14 human samples from the outbreak revealed the presence of 3 dissimilar genotypes. Two genotypes were also present in livestock at 9 farms in the outbreak area. SNP analyses of bulk milk from 5 other farms, commercial cow milk, and cow colostrum revealed 2 additional genotypes that were not detected in humans. SNP genotyping data from clinical samples clearly demonstrate that at least 5 different C. burnetii genotypes are involved in the Dutch outbreak.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3067327 | PMC |
| http://dx.doi.org/10.1128/AEM.02293-10 | DOI Listing |
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