AI Article Synopsis
- A novel four-way ligation method was created to quickly build a full-length antibody library using a specialized mammalian expression vector.
- This vector included dual expression cassettes and specific sequences for unique restriction enzymes, allowing for efficient insertion of antibody genes.
- The technique enabled the display of complete bivalent antibodies on cell surfaces and significantly reduced the construction time to just two weeks.
Article Abstract
A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one step by four-way ligation, and full-length bivalent antibodies were displayed on mammalian cell surfaces. Using this strategy, only 2 weeks were required to successfully construct high-quality, full-length human antibody libraries.
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| http://dx.doi.org/10.1093/abbs/gmq126 | DOI Listing |
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