A new chromatographic stationary phase obtained by coating a reversed phase amide column with phosphatidylcholine based liposomes solution to yield a phospholipid modified column (PLM). The modification is achieved by the dynamic coating method which recycles the coating solution through the column in a closed loop for a period of 24 h. The chromatographic properties of the new column have changed significantly as compared to the original amide column due to the phospholipid coating. A good correlation was observed between n-octanol/water logP values and the logarithm of the retention factor obtained on the PLM column for a large number of solutes. In addition the PLM column was characterized using the linear solvation energy relationship (LSER). The values of the LSER system constants for the PLM column were calculated and were found to be very close to those of the n-octanol/water extraction system thus suggesting that the PLM column can be used for the estimation of n-octanol/water partition coefficient and serve as a possible alternative to the shake-flask method for lipophilicity determination. In addition, the results suggest that the PLM column can provide an alternative to other phospholipid-based column such as the IAM and the DPC columns.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2010.12.105 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Privacy-preserving for assembly deviation prediction in a machine learning model of hydraulic equipment under value chain collaboration.
Sci Rep
June 2022
State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310027, People's Republic of China.
Hydraulic equipment, as a typical mechanical product, has been wildly used in various fields. Accurate acquisition and secure transmission of assembly deviation data are the most critical issues for hydraulic equipment manufacturer in the PLM-oriented value chain collaboration. Existing deviation prediction methods are mainly used for assembly quality control, which concentrate in the product design and assembly stage.
View Article and Find Full Text PDFDesign of Macroscopically Ordered Liquid Crystalline Hydrogel Columns Knitted with Nanosilver for Topical Applications.
Bioconjug Chem
April 2017
Chemical Sciences and Technology Division and ‡Agro-Processing and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019, India.
The design of liquid crystalline hydrogels knitted with silver nanoparticles in macroscopic ordering is becoming a subject of research interest due to their promising multifunctional applications in biomedical and optoelectronic applications. The present work describes the development of liquid crystalline Schiff-based hydrogel decorated with silver nanoparticles and the demonstration of its antifungal applications. Schiff base was prepared from polyglucanaldehyde and chitosan, and the former was prepared by the oxidation of amylose (polyglucopyranose) isolated from abundantly available unutilized jackfruit seed starch.
View Article and Find Full Text PDFCCMpred--fast and precise prediction of protein residue-residue contacts from correlated mutations.
Bioinformatics
November 2014
Gene Center, LMU Munich, Feodor-Lynen-Strasse 25, 81377, Munich and Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany Gene Center, LMU Munich, Feodor-Lynen-Strasse 25, 81377, Munich and Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Motivation: Recent breakthroughs in protein residue-residue contact prediction have made reliable de novo prediction of protein structures possible. The key was to apply statistical methods that can distinguish direct couplings between pairs of columns in a multiple sequence alignment from merely correlated pairs, i.e.
View Article and Find Full Text PDFZebrafish hoxd4a acts upstream of meis1.1 to direct vasculogenesis, angiogenesis and hematopoiesis.
PLoS One
October 2013
School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Mice lacking the 4th-group paralog Hoxd4 display malformations of the anterior vertebral column, but are viable and fertile. Here, we report that zebrafish embryos having decreased function of the orthologous hoxd4a gene manifest striking perturbations in vasculogenesis, angiogenesis and primitive and definitive hematopoiesis. These defects are preceded by reduced expression of the hemangioblast markers scl1, lmo2 and fli1 within the posterior lateral plate mesoderm (PLM) at 13 hours post fertilization (hpf).
View Article and Find Full Text PDFCoronatin-1 isolated from entomopathogenic fungus Conidiobolus coronatus kills Galleria mellonella hemocytes in vitro and forms potassium channels in planar lipid membrane.
Toxicon
September 2011
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warszawa, Poland.
Entomopathogenic fungi are important natural regulatory factors of insect populations and have potential as biological control agents of insect pests. The cosmopolitan soil fungus Conidiobolus coronatus (Entomopthorales) easily attacks Galleria mellonella (Lepidoptera) larvae. Prompt death of invaded insects is attributed to the action of toxic metabolites released by the invader.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!