Fluorochrome-labeled oligonucleotides (n = 44) corresponding to mouse genome repetitive sequences were hybridized in situ with pachytene nuclei of mouse spermatocytes. Signals of the repetitive sequences MaLR, MER, and (GT)22 were found to be dispersed through chromatin, and signals of BI 1 repeats and minisatellites were mostly attached to synaptonemal complexes immunostained with anti-SYCP3 antibodies. These results suggest that B 1 repeats and minisatellites are candidates for sequences anchoring chromatin to synaptonemal complexes.
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We lack tools to edit DNA sequences at scales necessary to study 99% of the human genome that is noncoding. To address this gap, we applied CRISPR prime editing to insert recombination handles into repetitive sequences, up to 1697 per cell line, which enables generating large-scale deletions, inversions, translocations, and circular DNA. Recombinase induction produced more than 100 stochastic megabase-sized rearrangements in each cell.
View Article and Find Full Text PDFNaturwissenschaften
January 2025
Department of Biology, University of Washington, Seattle, WA, 98195, USA.
Four main classes of introns (group I, group II, spliceosomal, and archaeal) have been reported for all major types of RNA from nuclei and organelles of a wide range of taxa. When and how introns inserted within the genic regions of genomes, however, is often unclear. Introns were examined from Archaea, Bacteria, and Eukarya.
View Article and Find Full Text PDFFront Cell Infect Microbiol
January 2025
College of Public Health, North China University of Science and Technology, Tangshan, China.
Enterohemorrhagic (EHEC) is a contagious foodborne pathogen that specifically colonizes the human large intestine, which is regulated by different environmental stimuli within the gut. Transcriptional regulation of EHEC virulence and infection has been extensively studied, while the posttranscriptional regulation of these processes by small RNAs (sRNAs) remains not fully understood. Here we present a virulence-regulating pathway in EHEC O157:H7, in which the sRNA EvrS binds to and destabilizes the mRNA of Z2269, a novel transcriptional regulator.
View Article and Find Full Text PDFBehav Processes
January 2025
Surrey Sleep Research Centre, University of Surrey.
Visuospatial working memory (VSWM) is crucial for navigating complex environments and is known to decline with ageing. The Free-Movement Pattern (FMP) Y-maze, used in animal studies, provides a robust paradigm for assessing VSWM via analyses of individual differences in repeated alternating sequences of left (L) and right (R) responses (LRLR, etc.), the predominant search pattern in many species.
View Article and Find Full Text PDFGenome
January 2025
USDA-ARS, Wheat, Sorghum & Forage Research Unit, Lincoln, Nebraska, United States.
(2n=2x=14, genome SS) is a wild relative of wheat and a donor of useful traits for wheat improvement. Several whole-genome studies compared genic regions of from the section and wheat and found that is most closely related to the wheat B subgenome but is not its direct progenitor. The results showed that a B subgenome ancestor diverged from more than 4 MYA and either has not yet been discovered, or is extinct.
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