Background: The Mirasol pathogen reduction technology system for plasma is based on a riboflavin and UV light treatment process resulting in pathogen inactivation due to irreversible, photochemically induced damage of nucleic acids. The objective of this study was to evaluate the in vitro protein quality of previously frozen FFP, thawed, Mirasol-treated and refrozen for a final storage period of up to 2 years at -30 °C.
Materials And Methods: All plasma products were held at 4 ± 2 °C, and frozen within 8h after the start of collection. FFP was stored frozen at -30°C for eight time intervals before riboflavin and UV light treatment and refrozen to -30 °C and stored for varying time intervals for a total storage period of up to 2 years at -30 °C. Results were compared to paired, untreated units thawed and stored for the same time intervals.
Results: The overall mean values for all time points in riboflavin and UV light treated plasma samples indicates that all proteins were well preserved following a period of frozen storage for 2 years at -30 °C. Factors V, VII and XI, retained 70%, 65% and 53% activity, respectively. All other protein factors, anticoagulant and inhibitor proteins demonstrated retention between 74% and 100%.
Conclusion: Riboflavin and UV light-treated FFP maintained both coagulant and anticoagulant in vitro protein quality after double freeze/thaw storage at -30 °C for up to 2 years, a finding that may offer processing flexibility to blood centers.
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http://dx.doi.org/10.1016/j.transci.2010.12.005 | DOI Listing |
Molecules
January 2025
Department of Environment and Agricultural Natural Resources, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi Arabia.
Two activated carbons were synthesized from baobab seeds (BSs) using two activators, sulfuric acid (BS-AAC) and sodium hydroxide (BS-BAC), for dye removal from aqueous solutions. Malachite green (MG) was used as a model dye. SEM, FTIR, TGA, and surface area were used to characterize the feedstock and synthesis activated carbons.
View Article and Find Full Text PDFAnimals (Basel)
January 2025
Sino-US Joint Lab on Nutrition and Metabolism of Ruminant, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
(Objectives) The objectives of this study were to evaluate the effect of half-replacement of the supplementary sulfate sources of Cu, Mn, and Zn with methionine-hydroxy-analog-chelated (MHAC) mineral or amino-acid-complexed (AAC) mineral forms in diets on the mineral status, blood immune biomarkers, and lameness of lactating cows. (Methods) Sixty multiparous Holstein cows (158 ± 26 days in milk; body weight: 665 ± 52 kg; milk yield: 32 ± 7 kg/day) were randomly assigned into one of three dietary treatments ( = 20 per group): (1) MHAC: 50% replacement of sulfate minerals with MHAC forms. (2) AAC: 50% replacement of sulfate minerals with AAC forms.
View Article and Find Full Text PDFActa Neurochir (Wien)
January 2025
Department of Orthopaedic Surgery, Seoul National University College of Medicine, SMG-SNU Boramae Medical Center, 20 Boramae-Ro 5-Gil, Dongjak-Gu, Seoul, Republic of Korea.
Background: The degenerative spondylosis can cause the difficulty in maintaining sagittal and coronal alignment of spine, and X-ray parameters are the gold standard to analyze the malalignment. This study aimed to develop a new 3D full body scanner to analyze the spinal balance and compare it to X-ray parameters.
Methods: Ninety-seven adult participants who suffer degenerative spondylosis underwent 3D full body scanning, whole spine X-rays, clinical questionnaires and body composition analyses.
J Bone Miner Res
January 2025
Departments of Medicine and Radiology, University of Manitoba, Winnipeg, Canada.
Vertebral fracture assessment (VFA) images from bone density machines enable the automated machine learning assessment of abdominal aortic calcification (ML-AAC), a marker of cardiovascular disease (CVD) risk. The objective of this study was to describe the risk of a major adverse cardiovascular event (MACE, from linked health records) in patients attending routine bone mineral density (BMD) testing and meeting specific criteria based on age, BMD, height loss, or glucocorticoid use have a VFA in the Manitoba Bone Mineral Density Registry. The cohort included 10 250 individuals (mean 75.
View Article and Find Full Text PDFMymensingh Med J
January 2025
Dr Parvez Arafa, Lecturer, Department of Virology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:
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