[Studies on separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin].

Objective: To study the technology for separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin.

Methods: The absorption and separation abilities of 10 kinds of adsorption resin were studied and the separation and purification technological process of phenylethanoid glycosides from leaves of Forsythia suspensa was investigated by HPLC with the content of Forsythoside A as an index.

Results: The optimal conditions were as follows: taking AB-8 resin column as adsorbent, the resin was washed by 20BV distilled water to remove impurity and 8BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2BV/h, the content of Forsythoside A in the extract was 34.8%.

Conclusion: The process with AB-8 resin is an effective method to separate and purify phenylethanoid glycosides.