We propose and tested a method for studies of native blood cells by atomic-force microscopy in a humid chamber preserving viability, size, and shape of biological objects. The method has some advantages over scanning in a liquid cell: it allows studying non-fixed blood samples in the form of suspension of live cells and excludes mechanical and chemical influences on the cells.
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http://dx.doi.org/10.1007/s10517-010-1121-y | DOI Listing |
Background: Currently there is no way to determine if archived cerebrospinal fluid (CSF) specimens have been properly handled and can be considered suitable for research purposes. Transthyretin (TTR) is abundant in CSF and undergoes a redox reaction that shifts its native proteoform into an S-cysteinylated form. This reaction proceeds spontaneously ex vivo when CSF is thawed, but ceases at storage temperatures of -80°C.
View Article and Find Full Text PDFBackground: Alzheimer's Disease (AD) and traumatic brain injuries (TBI) are frequently associated in medical literature, with a significant prevalence of TBI history observed among individuals diagnosed with AD. Our investigation focuses on this intersection, explicitly examining the risk of AD in individuals with a history of TBI. While current targets in cerebrospinal fluid and plasma can effectively detect acute TBI, the challenge lies in identifying biosignatures associated with TBI long after injury.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Background: Cardiometabolic disorders are emerging risk factors for Alzheimer's disease (AD) and AD-related dementia (ADRD). There is currently insufficient understanding of how different cardiometabolic profiles and blood biomarkers impact different AD-related brain pathology regionally. This project uses data-driven approaches and explainable artificial intelligence methods to determine the cardiometabolic and fluid contributions toward AD-related pathophysiologic patterns in the brain.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
D'Or Institute for Research and Education, Rio de Janeiro, RJ, Brazil.
Background: Dementia is a growing concern throughout the developing world and is severely underdiagnosed among the Brazilian population. Despite remarkable progress in the biomarker field in recent years, local testing and validation of plasma biomarkers of AD and dementia is still lacking in Brazil and Latin America.
Method: In this longitudinal cohort study of 145 participants, the diagnostic performance of plasma biomarkers was assessed based on clinical diagnosis and CSF biomarker positivity.
Alzheimers Dement
December 2024
Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Background: Cardiometabolic disorders are emerging risk factors for Alzheimer's disease (AD) and AD-related dementia (ADRD). There is currently insufficient understanding of how different cardiometabolic profiles and blood biomarkers impact different AD-related brain pathology regionally. This project uses data-driven approaches and explainable artificial intelligence methods to determine the cardiometabolic and fluid contributions toward AD-related pathophysiologic patterns in the brain.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!