AI Article Synopsis
- The study introduces an enhanced cytotoxicity test using S9 microsomal fractions encapsulated in alginate gel microbeads to minimize toxic effects from lipid peroxides produced during the testing process.
- The S9-encapsulated microbeads, created with a coaxial nozzle and coated in poly-L-lysine, showed a slower metabolic rate compared to unencapsulated S9 but successfully retained harmful byproducts.
- Testing revealed that the cytotoxicity of the mutagen cyclophosphamide was significantly reduced in the presence of the encapsulated microbeads, allowing for more accurate assessments of reactive metabolite toxicity.
Article Abstract
We present an improved cytotoxicity test for reactive metabolites, in which the S9 microsomal fraction of rat liver homogenate is encapsulated in alginate gel microbeads to avoid cytotoxic effects of S9-self-generated toxicants, microsomal lipid peroxides. The S9-encapsulated gel microbeads were prepared by a coaxial two-fluid nozzle and surfaces of the microbeads were coated with poly-L-lysine (PLL). Although the initial metabolic rate of the S9-encapsulated gel microbeads was about 20% slower than that of bare S9, the microbeads prevented the leakage of microsomal lipid peroxides thanks to the dense alginate and PLL polymer networks. In fact, the half maximal effective concentration of the indirect mutagen cyclophosphamide on NIH3T3 cells in the presence of the S9-encapsulated gel microbeads was about 5 times higher than that in the presence of bare S9. Use of the S9-encapsulated gel microbeads enabled the more accurate evaluation of the cytotoxicity of the reactive metabolites without the S9-based cytotoxicity.
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| http://dx.doi.org/10.1016/j.jbiosc.2010.12.004 | DOI Listing |
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