Background: We previously showed that meconium causes lung cell death by apoptosis and inflammatory cytokine expression. Whether this is due to meconium exposure itself, or meconium related hypoxia remains unclear.
Objectives: To elucidate the effects of meconium, saline, milk, hypoxia and hyperoxia induced lung injury.
Design/methods: We studied 5 groups of rabbit pups: (I) normal saline; (II) Milk; (III) 10% solution of meconium; (IV) only to 15 minutes of hypoxia (10% O(2)), and (V) 5 minutes of hypoxia (95% O(2)). After exposure lung lavage cells were used for apoptotic cell count and cytokine expression. In vitro response of human A 549 epithelial cells to meconium-and milk exposure was also studied.
Results: There was no difference in cell death between saline and milk groups. However, meconium caused a significant cell loss compared to saline and milk-Inflammatory cytokines increased significantly in meconium group compared to saline or milk group. Although hypoxic and hyperoxic lungs showed increased inflammatory reaction compared to saline-treated lungs, this injury was not significant compared to meconium group. Studies with A549 cells also showed similar results.
Conclusions: We conclude that lung cell injury in meconium aspiration is mainly from meconium itself.
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| http://dx.doi.org/10.1155/2010/204831 | DOI Listing |
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