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Formation of epithelial polarity on the fluorinated-oil microdroplet surface by regulating cell adhesion.
J Biosci Bioeng
January 2025
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Polarized epithelial cells are compartmentalized into apical and basement membranes with asymmetrically distributed proteins. This study aimed to establish a method for culturing epithelial cells at the fluorinated oil (Novec-7500) microdroplet surface for the formation of epithelial polarity, which is desirable for regenerative medicine and drug discovery research. Microdroplet surfaces treated with fibronectin, which regulates a variety of cell behaviors through direct interactions with cell surface integrin receptors, were prepared for culturing epithelial cells.
View Article and Find Full Text PDF"The most exciting thing about my research is exploring the remarkable catalytic potential of enzymes and witnessing the passion and motivation in the eyes of students and young co-workers, as they engage with scientific discovery… My first experiment was developing analog photographs in a darkroom I set up in the basement of my parents' house…" Find out more about Paweł Borowiecki in his Introducing… Profile.
View Article and Find Full Text PDFMineralization and metallogenic model of the Laurani high-sulfidation epithermal deposit in northeastern Bolivian Altiplano.
Sci Rep
December 2024
College of Mining Engineering, Guizhou University of Engineering Science, Bijie, 551700, China.
The Laurani high-sulfidation epithermal deposit, located in the northeastern Altiplano of Bolivia, is a representative gold-polymetallic deposit linked to the late Miocene volcanic rocks that were formed approximately at about 7.5 Ma. At Laurani, four mineralization stages are defined.
View Article and Find Full Text PDFRadiation-induced nephrotoxicity: Role of SMPDL3b.
Int J Radiat Oncol Biol Phys
December 2024
Department of Radiation Oncology, University of Miami, Sylvester Comprehensive Cancer Center/ Miller School of Medicine, Miami, FL, USA; Department of Radiation Oncology, University of Rochester, 601 Elmwood Ave. Box 647 Rochester, NY, USA. Electronic address:
Background: Radiation nephropathy (RN) can be a significant late complication after radiotherapy for abdominal and paraspinal tumors. The mechanisms for the development of RN are thought to involve disruption of podocyte function, leading to podocyte cell death and, finally, impaired renal function. This study investigated the mechanistic role of SMPDL3b in regulating podocyte injury and renal function after irradiation.
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