Background: The presence of chicken group-IIA PLA2 (ChPLA2-IIA) in the intestinal secretion suggests that this enzyme plays an important role in systemic bactericidal defence. We have analyzed the bactericidal activity of purified ChPLA2-IIA, on several gram-positive and gram-negative bacteria by using the diffusion well and dilution methods.
Results: ChPLA2-IIA displays potent bactericidal activity against gram-positive bacteria but lacks bactericidal activity against gram negative ones. We have also demonstrated a synergic action of ChPLA2-IIA with lysozyme when added to the bacteria culture prior to ChPLA2-IIA. The bactericidal efficiency of ChPLA2-IIA was shown to be dependent upon the presence of calcium ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Interestingly ChPLA2-IIA displays a higher dependence to Ca²+ ions than to Mg²+ ions.
Conclusion: We conclude that the main physiological role of ChPLA2-IIA could be the defence of the intestine against bacterial invasions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024238 | PMC |
| http://dx.doi.org/10.1186/1476-511X-10-4 | DOI Listing |
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