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Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems.
Ecotoxicol Environ Saf
December 2024
Department of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, China.
In this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes revealed Norovirus as having the highest infection rate, followed by Adenovirus Group F and Rotavirus Group A. Despite not testing for Astrovirus in clinical labs, its sewage detection rate was surpassed only by Norovirus, suggesting a potentially high clinical infection rate.
View Article and Find Full Text PDFEvaluating the capacity of magnetic ionic liquids for separation and concentration of non-enveloped viral particles and free viral genomic RNA.
Anal Bioanal Chem
January 2025
Department of Food Science, University of Massachusetts, Amherst, MA, USA.
Magnetic ionic liquids (MILs) have proven effective as capture reagents for foodborne bacterial pathogens; however, there are currently no published studies regarding their use with foodborne, non-enveloped viruses. In this study, a protocol was evaluated for capture and recovery of bacteriophage MS2, a human norovirus surrogate, and purified viral genomic single stranded RNA (ssRNA) from an aqueous suspension using MILs. Transition metal-based MILs showed similar capture and recovery efficiency for both targets.
View Article and Find Full Text PDFEstablishment and characterization of noro-VLP measurement by digital ELISA.
Anal Methods
October 2024
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
Highly sensitive viral analytical techniques are essential tools for preventing the spread of infections. In this study, we established a digital enzyme-linked immunosorbent assay (ELISA) system to quantify norovirus proteins with high sensitivity. We used norovirus-like particles (noro-VLPs) as a surrogate for norovirus and constructed two digital ELISA systems using two different antibody pairs.
View Article and Find Full Text PDFUltraviolet (UV-C) Light Systems for the Inactivation of Feline Calicivirus and Tulane Virus in Model Fluid Foods.
Food Environ Virol
December 2024
Department of Food Science, University of Tennessee, 2600 River Drive, Knoxville, TN, 37996, USA.
Conventional UV-C (254 nm) inactivation technologies have limitations and potential operator-safety risk. To overcome these disadvantages, novel UV-C light-emitting diodes (LED) are developed and investigated for their performance. This study aimed to determine the inactivation of human norovirus (HuNoV) surrogates, Tulane virus (TV), and feline calicivirus (FCV-F9), by UV-C (254 nm) in comparison to UV-C LED (279 nm) in phosphate-buffered saline (PBS) and coconut water (CW).
View Article and Find Full Text PDFElectroless silver plating on fabrics for antimicrobial coating: comparison between cotton and polyester.
J Appl Biomater Funct Mater
October 2024
Institute of Clinical Physiology, National Research Council, Massa, Italy.
In the past few years, due to the Covid-19 pandemic, the interest towards textiles with antimicrobial functionalities faced a significant boost. This study proposes a rapid and convenient method, in terms of reactants and equipment, for fabricating antimicrobial coatings on textiles. Through the electroless silver plating reaction, silver coatings were successfully applied on cotton and polyester, rapidly and at room temperature.
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