The effects of the caprine α s1-casein (CSN1S1) polymorphisms on milk quality and cheese yield have been widely studied in French and Italian goat breeds. Much less is known about the consequences of κ-casein (CSN3) genotype on the technological and coagulation properties of goat milk. In the current study, we have performed an association analysis between polymorphisms at the goat CSN1S1 and CSN3 genes and milk coagulation (rennet coagulation time, curdling rate and curd firmness) and technological (time to cutting of curd and cheese yield) properties. In this analysis, we have included 193 records from 74 Murciano-Granadina goats (with genotypes constituted by different combinations of alleles B, E and F of the gene CSN1S1 and alleles A and B of the gene CSN3) distributed in three herds, which were collected bimonthly during a whole lactation. Data analysis, using a linear mixed model for repeated observations, revealed significant associations between CSN1S1 genotypes and the rate of the curdling process. In this way, milk from EE goats had a significantly higher curdling rate than milk from BB individuals (P<0·05). Contrary to previous experiments performed in French breeds, cheese yield was not significantly different in BB, EE and EF goats. Moreover, we have shown that CSN3 genotype has a significant effect on the rennet coagulation time (BB>AB, P<0·05) but not on cheese yield. No interaction between the CSN1S1 and CSN3 genotypes was observed.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1017/S002202991000083X | DOI Listing |
Animals (Basel)
August 2024
Equus Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
To investigate molecular regulation involved in lactation during pregnancy, this study focused on the transcriptomic profiles of mammary tissue from lactating and non-lactating Mongolian mares at the second month of gestation. A total of 4197 differentially expressed genes were identified by comparing mammary tissues from pregnant mares at two different states, including 1974 differentially expressed genes such as the milk protein-related genes a-s1-casein (), k-casein (), lactalbumin (), and lactoferrin (), which were highly expressed in the lactating mares group, and overall, these differentially expressed genes were mainly associated with biological processes such as endoplasmic reticulum protein processing, the Toll-like receptor signaling pathway, steroid biosynthesis, cytokine-cytokine receptor interactions, and amino sugar and nucleotide glycolysis. These findings serve as a foundation for investigating the molecular underpinnings of lactation in pregnant equids.
View Article and Find Full Text PDFJ Dairy Sci
December 2024
School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061. Electronic address:
Cow milk is rich in protein. Major cow milk proteins include α casein (CSN1S1), α casein (CSN1S2), β casein (CSN2), κ casein (CSN3), lactalbumin α (LALBA), and β-LG. These milk proteins are produced through gene expression in the mammary epithelial cells.
View Article and Find Full Text PDFJ Agric Food Chem
May 2024
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
The gene encodes a Na and pH-independent transporter protein that regulates cell growth by regulating the uptake of AA. This study, utilizing RNA-seq, aimed to explore the effect of on the synthesis of milk proteins and fats in goat mammary epithelial cells (GMECs) through gene interference and overexpression techniques. The results demonstrated that the overexpression of resulted in a significant increase in the expression of , , , , α-casein, p-S6K, and p-S6.
View Article and Find Full Text PDFAnim Biotechnol
November 2024
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
The lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.
View Article and Find Full Text PDFJ Dairy Sci
August 2023
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala 750 07, Sweden.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!