AI Article Synopsis
- A hybrid chip made of polymer and stainless steel is designed for efficient electroporation of cells in suspension, featuring parallel stainless steel mesh electrodes to ensure uniform flow and electroporation.
- The device achieved successful electroporation of 20 million human dendritic cells with mRNA, offering similar performance to traditional methods without limiting cell quantity.
- Its design allows for easy integration with other microfluidic components and enables cost-effective mass production using injection-molded polymer and standard stainless steel mesh.
Article Abstract
We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10544-010-9507-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material.
J Vis Exp
December 2024
School of Engineering and Materials Science, Queen Mary University of London.
Under current minimally invasive treatment regimes, minor tooth preparation and thinner biomimetic ceramic restoration are used to preserve the restored tooth's vitality, aesthetics, and function. New computer-aided design and computer-aided manufacturing (CAD/CAM) ceramic-like material are now available. To guarantee longevity, a dental clinician must know these newly launched product's mechanical strength compared to the relatively brittle glass-matrix ceramic.
View Article and Find Full Text PDFFacile synthesis of CoTe-FeC for efficient overall water-splitting in an alkaline medium.
Nanoscale Adv
December 2024
School of Electronics and Communication Engineering, Quanzhou University of Information Engineering Quanzhou Fujian China
The large amounts of attention directed towards the commercialization of renewable energy systems have motivated extensive research to develop non-precious-metal-based catalysts for promoting the electrochemical production of H and O from water. Here, we report promising technology, , electrochemical water splitting for OER and HER. This work used a simple hydrothermal method to synthesize a novel CoTe-FeC nanocomposite directly on a stainless-steel substrate.
View Article and Find Full Text PDFExperimental and numerical investigation of dissimilar weld characterization in laser welding of duplex 2205 and AISI 1060 steel: Microstructure, mechanical properties and weld geometry.
Heliyon
December 2024
Department of Mechanics, Electrical Power and Computer, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Dissimilar laser welding of martensitic AISI 1060 carbon steel and Duplex Stainless Steel 2205 was performed based on an experimental and numerical study. The experiments were then conducted based on central composite design experiments (CCD) and analyzed via the response surface methodology (RSM) by considering the effect of laser welding process parameters (incident laser power, speed of welding, nozzle distance and deviation of laser beam) on the weld joint characterization. The experimental results showed that the laser power had a remarkable effect on the melt pool depth.
View Article and Find Full Text PDFRice husk and lemongrass-derived eco-enzymes as potential food contact surface disinfectants against biofilm-forming foodborne pathogens.
FEMS Microbiol Lett
January 2025
School of Biotechnology, Mila University, No 1, MIU Boulevard, Putra Nilai, Nilai 71800, Malaysia.
This study aims to evaluate the rice husk (EE-R) and lemongrass (EE-L) derived-eco-enzymes (EE) as alternatives to chemical-based disinfectants. The EE-R and EE-L's antimicrobial activity were tested against Pseudomonas aeruginosa, Salmonella Typhimurium, and Staphylococcus aureus using a broth microdilution method. The antibiofilm activities of EE were determined using crystal violet staining.
View Article and Find Full Text PDFMultifactored accelerated marine corrosion of immersed steels influenced by washed ashore Sargassum rafts.
Mar Environ Res
December 2024
L3MA UR4_1 UFR STE Universite des Antilles, Campus de Schoelcher, Schoelcher, 97275, France. Electronic address:
Since 2011, massive strandings of Sargassum (brown alga) have significantly affected Caribbean islands causing major health, environmental and economic problems. Amongst them, the degradation of algae releases corrosive gases, hydrogen sulphide (HS) and ammonia (NH) which causes an accelerated corrosion of the metallic structures of these coastal areas. The aim of this study was to quantify the impact of Sargassum strandings on the corrosion of three types of steels (DC01 carbon steel, 304L and 316L stainless steels) immersed for up to 120 days at various sites in Martinique which were gradually impacted by Sargassum.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!