AI Article Synopsis
- Members of the Oxa1/Alb3/YidC family are crucial for inserting and assembling membrane proteins, with Bacillus subtilis having two similar proteins, SpoIIIJ and YqjG.
- While SpoIIIJ is essential for spore formation and has a unique role, YqjG provides complementary functions in membrane protein processes, especially when both proteins are deficient.
- The study reveals that without SpoIIIJ and YqjG, the assembly of ATP synthase is impaired, and it also identifies a specific role for YqjG in genetic competence development for the first time.
Article Abstract
Members of the evolutionary conserved Oxa1/Alb3/YidC family have been shown to play an important role in membrane protein insertion, folding and/or assembly. Bacillus subtilis contains two YidC-like proteins, denoted as SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable, but SpoIIIJ is essential for spore formation and YqjG cannot complement this activity. To elucidate the role of YqjG, we determined the membrane proteome and functional aspects of B. subtilis cells devoid of SpoIIIJ, YqjG or both. The data show that SpoIIIJ and YqjG have complementary functions in membrane protein insertion and assembly. The reduced levels of F(1)F(O) ATP synthase in cells devoid of both SpoIIIJ and YqjG are due to a defective assembly of the F(1)-domain onto the F(0)-domain. Importantly, for the first time, a specific function is demonstrated for YqjG in genetic competence development.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pmic.201000435 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!