One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co-expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3009751 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015801 | PLOS |
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