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Multiwavelength Optical Rotation Detection: An Effective Approach for the Recognition of Analytical and Semi-Preparative HPLC Enantioseparation of the Chiral Pheromone Olean and Its Stereochemical Characterization.
Chirality
November 2024
Centre for the Control and Evaluation of Medicines, Istituto Superiore di Sanità, Rome, Italy.
Olean is the chiral spiroacetal sex pheromone of the female olive fruit fly Bactrocera oleae. Laboratory tests have demonstrated that the (R)-(-)-olean enantiomer is active on males, whereas females respond to (S)-(+)-olean. Here we present the first HPLC enantioseparation of olean using polysaccharide derivatives as chiral stationary phases and a polarimetric detector equipped with a micro-flow cell capable of detecting optical rotation at six different wavelengths.
View Article and Find Full Text PDFDiffractive Beam Shaper for Multiwavelength Lasers for Flow Cytometry.
Cytometry A
February 2021
State Key Laboratory of Precision Measurement Technology and Instrument, Tsinghua University, Beijing, China.
Illumination spot in a flow cytometer is a crucial factor determining the measurement accuracy and stability. The traditional mechanism is to precisely calibrate multiple optical components to convert circular Gaussian beams into elliptical Gaussian beams, making it difficult to shape multiwavelength lasers simultaneously. A diffractive beam shaper for multicolor lasers with high simplicity, only containing one diffractive optical element and one focusing lens is created in this work.
View Article and Find Full Text PDFMulti-wavelength microflow cytometer using groove-generated sheath flow.
Lab Chip
July 2009
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
A microflow cytometer was developed that ensheathed the sample (core) fluid on all sides and interrogated each particle in the sample stream at four different wavelengths. Sheathing was achieved by first sandwiching the core fluid with the sheath fluid laterally via fluid focusing. Chevron-shaped groove features fabricated in the top and bottom of the channel directed sheath fluid from the sides to the top and bottom of the channel, completely surrounding the sample stream.
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