AI Article Synopsis
- The endoplasmic reticulum (ER) is crucial for synthesizing membrane and secretory proteins, but improper folding leads to ER stress.
- Recent studies have linked ER stress to various diseases, highlighting the need for better understanding through in vivo research.
- This report introduces Raman spectroscopy as a method to detect ER stress in living organisms, showing that specific Raman bands decrease in relation to ER stress, offering a non-transgenic approach for studying this condition.
Article Abstract
The endoplasmic reticulum (ER) is an organelle in which most membrane and secretory proteins are synthesized. If these proteins are not folded correctly, unfolded proteins accumulate in the ER lumen, causing a cellular situation known as ER stress. Recently, many studies on the relationship between ER stress and diseases have been reported. Thus, studies of ER stress in vivo should yield information that is useful in pathology. Model mice have been developed as a powerful tool to visualize ER stress in vivo, but this approach depends on transgenic technology. Here, we report on a method of detecting ER stress in vivo by Raman spectroscopy. Our experiments revealed that two specific Raman bands were reduced in both cultured cells and animal tissues in an ER stress dependent manner. This suggests that Raman spectroscopy could be a useful tool to detect ER stress in vivo without transgenic technology.
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| http://dx.doi.org/10.1016/j.bbrc.2010.12.112 | DOI Listing |
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