We describe methods of preparation of lipid membranes supported on optically transparent nanosilicas (20 and 30 nm diameter) and an initial application of these materials to characterize membrane-protein binding using conventional circular dichroism and fluorescence spectroscopy.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123449 | PMC |
| http://dx.doi.org/10.1016/j.jcis.2010.11.064 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Loading of Extracellular Vesicles with Nucleic Acids via Hybridization with Non-Lamellar Liquid Crystalline Lipid Nanoparticles.
Adv Sci (Weinh)
December 2024
Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, 8093, Switzerland.
The translation of cell-derived extracellular vesicles (EVs) into biogenic gene delivery systems is limited by relatively inefficient loading strategies. In this work, the loading of various nucleic acids into small EVs via their spontaneous hybridization with preloaded non-lamellar liquid crystalline lipid nanoparticles (LCNPs), forming hybrid EVs (HEVs) is described. It is demonstrated that LCNPs undergo pH-dependent structural transitions from inverse hexagonal (H) phases at pH 5 to more disordered non-lamellar phases, possibly inverse micellar (L) or sponge (L) phases, at pH 7.
View Article and Find Full Text PDFImproving rooster semen cryopreservation with telmisartan-enhanced extender.
Poult Sci
December 2024
Department of Poultry Science, University of Georgia, Athens, GA 30602-2772, USA.
Semen cryopreservation is essential for preserving genetic resources and enabling artificial insemination in poultry breeding. However, avian sperm is known to experience detrimental changes during the freezing process. Telmisartan, an angiotensin-II receptor antagonist recognized for its antioxidant properties and ability to activate AMP-activated protein kinase (AMPK), was hypothesized to improve post-thaw semen quality by enhancing mitochondrial function and providing antioxidant protection to sperm.
View Article and Find Full Text PDFBackground: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading pathogen causing severe endovascular infections. The prophage-encoded protein Gp05 has been identified as a critical virulence factor that contributes to MRSA persistence during vancomycin (VAN) treatment in an experimental endocarditis model. However, the underlining mechanisms driving this persistence phenotype remain poorly understood.
View Article and Find Full Text PDFThe Target of Rapamycin kinase is a positive regulator of plant fatty acid and lipid synthesis.
Plant Physiol
December 2024
Department of Biology, BNL 463, 50 Bell Ave, Upton NY 11973, USA.
In eukaryotes, Target of Rapamycin (TOR), a conserved protein sensor kinase, integrates diverse environmental cues, including growth factor signals, energy availability, and nutritional status, to direct cell growth. In plants, TOR is activated by light and sugars and regulates a wide range of cellular processes, including protein synthesis and metabolism. Fatty acid synthesis is key to membrane biogenesis that is required for cell growth.
View Article and Find Full Text PDFHigher-order transient membrane protein structures.
Proc Natl Acad Sci U S A
January 2025
Laboratory of Molecular Neurobiology and Biophysics, The Rockefeller University, New York, NY 10065.
This study shows that five membrane proteins-three GPCRs, an ion channel, and an enzyme-form self-clusters under natural expression levels in a cardiac-derived cell line. The cluster size distributions imply that these proteins self-oligomerize reversibly through weak interactions. When the concentration of the proteins is increased through heterologous expression, the cluster size distributions approach a critical distribution at which point a phase transition occurs, yielding larger bulk phase clusters.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!