The pancreatic zymogen granule membrane protein (GP2) is expressed by pancreatic acinar cells and M cells of the ileum. GP2 is the closest related homologue of the urine resident Tamm-Horsfall protein (THP). Recently, it was shown that THP is a ligand of various scavenger receptors (SRs). Therefore, we were interested, if GP2 has similar properties. cDNA of different SRs was stably transfected into a murine thymoma cell line. GP2 was recombinantly expressed, purified and biotinylated. Binding or uptake of GP2 by transfected cells or monocyte-derived dendritic cells (moDCs) was analyzed by flow-cytometry. GP2 is a binding partner of the scavenger receptor expressed on endothelial cells I (SREC-I) but not of SR-AI and SR-BI. The dissociation constant (K(d)) of GP2 binding to SREC-I is 41.3nM. SREC transfected cells are able to internalize GP2. moDCs express SREC-I and also bind and internalize GP2. Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding. Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response. In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040788 | PMC |
| http://dx.doi.org/10.1016/j.cellimm.2010.12.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Boosting skin cancer diagnosis accuracy with ensemble approach.
Sci Rep
January 2025
School of Information and Electronic Engineering and Zhejiang Key Laboratory of Biomedical Intelligent Computing Technology, Zhejiang University of Science and Technology, No. 318, Hangzhou, Zhejiang, China.
Skin cancer is common and deadly, hence a correct diagnosis at an early age is essential. Effective therapy depends on precise classification of the several skin cancer forms, each with special traits. Because dermoscopy and other sophisticated imaging methods produce detailed lesion images, early detection has been enhanced.
View Article and Find Full Text PDFVariable Assembly and Procapsid Binding of Bacteriophage P22 Terminase Subunits in Solution.
Pathogens
December 2024
Laboratory of Macromolecular Structure, Department of Molecular Biology and Biochemistry, University of California Irvine, Steinhaus Hall, Irvine, CA 92697-3900, USA.
Concatemeric viral DNA is packaged into bacteriophage P22 procapsids via a headful packaging mechanism mediated by a molecular machine consisting of small (gp3) and large (gp2) terminase subunits. Although a negative stain reconstruction exists for the terminase holoenzyme, it is not clear how this complex binds the dodecameric portal protein located at a 5-fold mismatch vertex. Herein, we describe new assemblies for the holoenzyme.
View Article and Find Full Text PDFParkinson's Disease Pathogenic Variants: Cross-Ancestry Analysis and Microarray Data Validation.
medRxiv
December 2024
Integrative Neurogenomics Unit (INU), Laboratory of Neurogenetics, National Institutes of Health, Bethesda, USA.
Background: Known pathogenic variants in Parkinson's disease (PD) contribute to disease development but have yet to be fully explored by arrays at scale.
Objectives: This study evaluated genotyping success of the NeuroBooster array (NBA) and determined the frequencies of pathogenic variants across ancestries.
Method: We analyzed the presence and allele frequency of 34 pathogenic variants in 28,710 PD cases, 9,614 other neurodegenerative disorder cases, and 15,821 controls across 11 ancestries within the Global Parkinson's Genetics Program dataset.
Machine learning-based estimation of crude oil-nitrogen interfacial tension.
Sci Rep
January 2025
Young Researchers and Elite Club, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran.
Accurate estimation of interfacial tension (IFT) between nitrogen and crude oil during nitrogen-based gas injection into oil reservoirs is imperative. The previous research works dealing with prediction of IFT of oil and nitrogen systems consider synthetic oil samples such n-alkanes. In this work, we aim to utilize eight machine learning methods of Decision Tree (DT), AdaBoost (AB), Random Forest (RF), K-nearest Neighbors (KNN), Ensemble Learning (EL), Support Vector Machine (SVM), Convolutional Neural Network (CNN) and Multilayer Perceptron Artificial Neural Network (MLP-ANN) to construct data-driven intelligent models to predict crude oil - nitrogen IFT based upon experimental data of real crude oils samples encountered in underground oil reservoirs.
View Article and Find Full Text PDFSynthesis And Photovoltaic Performance of Carbazole (Donor) Based Photosensitizers in Dye-Sensitized Solar Cells (DSSC): A Review.
Top Curr Chem (Cham)
December 2024
Department of Chemistry, King Khalid University, P.O. Box 9004, 61413, Abha, Saudi Arabia.
Carbazoles are nitrogen-containing aromatic heterocycles, having widespread applications in the field of photovoltaics. Carbazole-based photosensitizers have tunable features for absorption on semi-conductor (tellurium dioxide or zinc oxide) layers to create sufficient push-pull force in the conversion of sunlight into electrical energy, thus presenting as promising heterocyclic donor candidates to be used in dye-sensitized solar cells. For the synthesis of these dyes, various structural designs are available, namely, D-A, D-π-A, D-D-π-A, D-A-π-A, A-π-D-π-A-π-A, and D2-π-A that all involve incorporating carbazole as a donor (D), along with spacer (π-extender) moieties, such as thiophene, phenol, ethynylene, nitromethane, azine, thiadiazole, or acetonitrile.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!