[Proteomic analysis of myocardial hypertrophy induced by left kidney artery coarctation in rats].

Aim: To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation.

Methods: 16 male SD rats were separated into 2 groups (n=8): 2 kidney 1 Clip group (2K1C) and sham operation group (SO). The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

Results: Overall, 21 protein spots showed significant difference, and 14 out of which were identified.

Conclusion: Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.