AI Article Synopsis
- A new analytical method for detecting two strains of influenza A viruses has been developed, utilizing nitrocellulose membranes and magnetic sensors with a unique frequency mixing technique.
- This method streamlines the detection process by eliminating the need for color development and reaction stop steps found in traditional methods like ELISA, resulting in a faster assay.
- The assay enables quantitative detection of viruses at very low concentrations (picograms), enhancing stability and repeatability for use in point-of-care applications.
Article Abstract
Here we describe a new analytical method for the detection of two influenza A viruses by nitrocellulose membrane and magnetic sensors that employ a special frequency mixing technique. The combination of the nitrocellulose membrane and magnetic bead detection permits a rapid assay procedure and excludes two steps (the development of color and the stop reaction) required for usual immunochemical detection methods such as ELISA. Quantitative virus detection was performed using magnetic beads conjugated with secondary antibody. The results were compared with conventional assay methods and with a dot-blot assay with fluorescence compound (FITC). Under optimum conditions, our new assay procedure is capable of detecting picograms of virus per well. This new method combining the nitrocellulose membrane and magnetic bead detection reduces analytical time and allows stable and repeatable analyses of samples in point-of-care applications.
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| http://dx.doi.org/10.1016/j.jim.2010.12.005 | DOI Listing |
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