[DNA barcoding in medicinal plants Caprifoliaceae].

Zhongguo Zhong Yao Za Zhi

Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China.

Published: October 2010

Objective: To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.

Method: PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.

Result: The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.

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