Expressed sequence tags (ESTs) are parts of complementary DNAs (cDNAs) with certain gene function.It provides us more information than other neutral markers. The association of EST markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. In this study, 12 candidate EST markers isolated from the cold-induced brain cDNA of common carp (Cyprinus carpio L.) were conducted the correlation analysis of marker and cold tolerance trait of common carp using GLM model of SPSS 17.0 software firstly, then tried to locate them in the genetic linkage map using OneMap software. As a result, eight out of 12 candidate EST makers were separately located in six linkage groups, in which marker CC009(P<0.05) and CC115 (P<0.01) were associated with cold tolerance and mapped to LG38 and LG2, respectively. Homology identity alignments showed that marker CC009 was highly homologous to the known Uridine-cytidine kinase I of Danio rerio with an identity of 94%, and marker CC115 was lowly homologous to the putative glycosyl transferase of Prochlorococcus marinus with an identity of 56%.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3724/SP.J.1141.2010.06581 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biomarker-Directed Radiotherapy in Breast Cancer: A Narrative Review.
JAMA Oncol
January 2025
Department of Radiation Oncology, Iridium Netwerk, Wilrijk-Antwerp, Belgium.
Importance: Integration of molecular biomarker information into systemic therapy has become standard practice in breast cancer care. However, its implementation in guiding radiotherapy (RT) is slower. Although postoperative RT is recommended for most patients after breast-conserving surgery and, depending on risk factors, following mastectomy, emerging evidence has indicated that patients with low scores on gene expression signatures or selected clinical-pathological features may have very low local recurrence rates.
View Article and Find Full Text PDFIdentifying biomarker-driven subphenotypes of cardiogenic shock: analysis of prospective cohorts and randomized controlled trials.
EClinicalMedicine
January 2025
University of Paris Cité, Inserm UMR-S 942, Cardiovascular Markers in Stress Conditions (MASCOT), Paris, France.
Background: Cardiogenic shock (CS) is a heterogeneous clinical syndrome, making it challenging to predict patient trajectory and response to treatment. This study aims to identify biological/molecular CS subphenotypes, evaluate their association with outcome, and explore their impact on heterogeneity of treatment effect (ShockCO-OP, NCT06376318).
Methods: We used unsupervised clustering to integrate plasma biomarker data from two prospective cohorts of CS patients: CardShock (N = 205 [2010-2012, NCT01374867]) and the French and European Outcome reGistry in Intensive Care Units (FROG-ICU) (N = 228 [2011-2013, NCT01367093]) to determine the optimal number of classes.
Deep analysis of the major histocompatibility complex genetic associations using covariate analysis and haploblocks unravels new mechanisms for the molecular etiology of Elite Control in AIDS.
BMC Immunol
January 2025
Laboratoire Génomique, Bioinformatique, et Chimie Moléculaire, Conservatoire National des Arts et Métiers, 2 rue Conté 75003, Paris, EA7528, France.
Introduction: We have reanalyzed the genomic data from the International Collaboration for the Genomics of HIV (ICGH), focusing on HIV-1 Elite Controllers (EC).
Methods: A genome-wide association study (GWAS) was performed, comparing 543 HIV-1 EC individuals with 3,272 uninfected controls (CTR) of European ancestry. 8 million single nucleotide polymorphisms (SNPs) and HLA class I and class II gene alleles were imputed to compare EC and CTR.
Screening Criteria Evaluation for Expansion in Pulmonary Neoplasias (SCREEN) II.
Can J Surg
January 2025
From the Faculty of Medicine, Dalhousie University, Halifax, N.S. (Huo); the Department of Primary Education, School of Education, University of Ioannina, Ioannina, Greece (Kontouli); the Department of Diagnostic Radiology, Dalhousie University, Halifax, N.S. (Manos); the Department of Pathology, Dalhousie University, Halifax, N.S. (Xu, Fris); the Department of Urology, Dalhousie University, Halifax, N.S. (Chun); the Division of Thoracic Surgery, Department of Surgery, Dalhousie University, Halifax, N.S. (Wallace, French)
Background: There is a need to expand eligibility criteria for lung cancer screening beyond age and smoking history. In this study, we sought to assess whether light-or-never-smokers and heavy smokers differ in molecular and immunologic markers based on conventional lung cancer screening criteria.
Methods: We conducted a retrospective review of lung cancer cases from 2005 to 2018 at a tertiary Canadian institution.
Extraction of 3D trajectories of mandibular condyles from 2D real-time MRI.
MAGMA
December 2024
IADI U1254, Inserm, Université de Lorraine, 54000, Nancy, France.
Objective: Computing the trajectories of mandibular condyles directly from MRI could provide a comprehensive examination, providing both anatomical and kinematic details. This study aimed to investigate the feasibility of extracting 3D condylar trajectories from 2D real-time MRI.
Materials And Methods: Twenty healthy subjects underwent real-time MRI while performing jaw opening and closing movements.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!