Aim: To investigate the protective effect of low molecular weight heparin (LMWH) on nephropathy in rats with pregnancy induced hypertension and to study its possible mechanism.
Methods: The levels of the expression of renal ERK1/2 protein and mRNA were detected in PIH rats which were made by injection of L-NAME, normal pregnant rats and rats treated with LMWH by immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction(RT-PCR). The renal tissue was observed by using light microscopy.
Results: The expression level of renal ERK1/2 protein and mRNA in LMWH-treated rats were significantly lower than that in PIH rats, while the expression level of renal ERK1/2 protein and mRNA in PIH rats was significantly higher than that in normal pregnant rats (P < 0.01), the intensity of ERK1/2 expression had no obvious differences among 3 groups. The average arterial pressure and urine protein in LMWH group were decreased, but no decrease was observed in normal rats. Mesangial expansion and basal membrane thickening were obviously retarded in LMWH- treated group.
Conclusion: LMWH has renal protective effect on PIH rats, whose mechanism may be associated partly with a down-regulation of ERK expression.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bile acids and the gut microbiome are involved in the hyperthermia mediated by 3,4-methylenedioxymethamphetamine (MDMA).
Sci Rep
June 2024
The Ohio Attorney General's Center for the Future of Forensic Science, Bowling Green State University, Bowling Green, OH, 43403, USA.
Hyperthermia induced by phenethylamines, such as 3,4-methylenedioxymethamphetamine (MDMA), can lead to life-threatening complications and death. Activation of the sympathetic nervous system and subsequent release of norepinephrine and activation of uncoupling proteins have been demonstrated to be the key mediators of phenethylamine-induced hyperthermia (PIH). Recently, the gut microbiome was shown to also play a contributing role in PIH.
View Article and Find Full Text PDFMicroRNA-195-5p Attenuates Pregnancy-Induced Hypertension by Inhibiting Oxidative Stress via OTX1/MAPK Signaling Pathway.
Biochem Genet
October 2024
Department of Gynecology and Obstetrics, Maternal and Child Health Hospital of Tangshan, No. 1 Hetai Road, Lunan District, Tangshan, 063000, China.
Pregnancy-induced hypertension (PIH) is a hypertensive disorder during pregnancy and can induce perinatal death of human infants. MicroRNA (miR)-195-5p was validated to display low expression in severe preeclampsia placentas, but the role of miR-195-5p in pregnancy-induced hypertension (PIH) has not been investigated. The study emphasized on the functions and mechanism of miR-195-5p in PIH.
View Article and Find Full Text PDF[Effects and mechanism of annexin A1-overexpressing human adipose-derived mesenchymal stem cells in the treatment of mice with acute respiratory distress syndrome].
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
May 2023
Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China.
To explore the effects and mechanism of annexin A1 ()-overexpressing human adipose-derived mesenchymal stem cells (AMSCs) in the treatment of mice with acute respiratory distress syndrome (ARDS). The experimental study method was adopted. After the adult AMSCs were identified by flow cytometry, the 3 passage cells were selected for the follow-up experiments.
View Article and Find Full Text PDF[Effects and mechanism of glycine on rat cardiomyocytes pretreated with serum from burned rats].
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
May 2023
Clinical Medical Research Center, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China.
To investigate the effect and mechanism of glycine on rat cardiomyocytes pretreated with serum from burned rats (hereinafter referred to as burn serum). Experimental research methods were adopted. Thirty gender equally balanced Wistar rats aged 7 to 8 weeks were collected, 10 of which were used to prepare normal rat serum (hereinafter referred to as normal serum), and the other 20 were inflicted with full-thickness burn of 30% total body surface area to prepare burn serum.
View Article and Find Full Text PDFRepeated episodes of postictal hypoxia are a mechanism for interictal cognitive impairments.
Sci Rep
September 2023
Cumming School of Medicine, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, T2N 4N1, Canada.
Comorbidities during the period between seizures present a significant challenge for individuals with epilepsy. Despite their clinical relevance, the pathophysiology of the interictal symptomatology is largely unknown. Postictal severe hypoxia (PIH) in those brain regions participating in the seizure has been indicated as a mechanism underlying several negative postictal manifestations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!