Evaluating age-associated phenotypes in a mouse model of protein dyshomeostasis.

Methods

McAllister Heart Institute, University of North Carolina, Chapel Hill, NC 27599, USA.

Published: March 2011

Proteotoxicity caused by an imbalanced protein quality control surveillance system is believed to contribute to the phenotypes associated with aging as well as many neurodegenerative diseases. Understanding and monitoring the impact of proteotoxicity in these processes offers researchers keen insight into the biology of aging, as well as other conditions that share similar pathological etiologies. In Section 2, we present various technical approaches that can be used to calculate and characterize the phenotypes associated with aging that are linked to increased proteotoxicity. Methods such as the measurement of oligomer protein expression and the capacity of proteasome function are useful tools in observing both aging phenotypes and neurodegenerative diseases, both of which share the phenomenon of impaired protein homeostasis.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057337PMC
http://dx.doi.org/10.1016/j.ymeth.2010.12.012DOI Listing

Publication Analysis

Top Keywords

phenotypes associated
8
associated aging
8
aging well
8
neurodegenerative diseases
8
evaluating age-associated
4
phenotypes
4
age-associated phenotypes
4
phenotypes mouse
4
mouse model
4
protein
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!