Background: While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.
Methods: Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.
Results: A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro. However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.
Conclusions: Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability.
Download full-text PDF |
Source |
---|
Heliyon
December 2024
Laboratoire Eaux Hydro-Systèmes et Agriculture (LEHSA), Institut International d'ingénierie de l'Eau et de l'Environnement (2iE), 1 Rue de la science 01 BP 594 Ouagadougou 01, Burkina Faso.
Sugarcane industries, like other agro-food industries, generate significant volumes of wastewater containing high concentrations of organic and inorganic pollutants. Among the proposed treatment solutions, the anaerobic membrane bioreactor (AnMBR) has proven highly effective in degrading organic pollutants but has limitations in removing color and inorganic pollutants. To address this gap, integrating other technologies with AnMBR is necessary.
View Article and Find Full Text PDFBMC Glob Public Health
December 2024
Emerging Diseases Epidemiology Unit, Institut Pasteur, Université Paris Cité, 25 Rue du Docteur Roux, 75015, Paris, France.
Background: Important differences in excess mortality between European countries during the COVID-19 pandemic have been reported. Understanding the drivers of these differences is essential to pandemic preparedness.
Methods: We examined patterns in age- and sex-standardized cumulative excess mortality in 13 Western European countries during the first 30 months of the COVID-19 pandemic and the correlation of country-level characteristics of interest with excess mortality.
Environ Res
December 2024
School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 2W2, Canada.
Microalgae-bacterial granular sludge (MBGS) process has great potential in achieving carbon neutrality and energy neutrality, but rapidly cultivating MBGS remains challenging. To address this challenge, this study proposes a new strategy to develop MBGS systems using pre-made granules from microalgae and dewatered sludge. The results indicate that using pre-made microalgae-dewatered sludge granules (M-DSG) as inoculants can directly develop MBGS system, with M-DSG maintaining a relatively stable granular structure, and ultimately achieving pollutant removal efficiencies of 94.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address:
Effects of incorporating sugar beet pectin (SBP) at various pH levels (3.0-7.0) on the conformation, interfacial characteristics, and emulsification performance of hempseed protein hydrolysate (HPH) were explored.
View Article and Find Full Text PDFBiofabrication
November 2024
Medical Engineering, Southeast University, Nanjing, Nanjing, 210096, CHINA.
Three-dimensional (3D) organotypic skin in vitro has attracted increasing attention for drug development, cosmetics evaluation, and even clinical applications. However, the severe contraction of these models restricts their application, especially in the analyses based on barrier functions such as percutaneous penetration. For the full-thickness skin equivalents, the mechanical properties of the dermis scaffold plays an important role in the contraction resistance.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!