AI Article Synopsis
- Glutathione S-transferases (GSTs) are a key gene family involved in insecticide resistance, with specific focus on six BmGST genes in Bombyx mori larvae.
- A real-time quantitative RT-PCR technique was utilized to assess gene expression levels in different larval tissues and their responses to insecticides and fluoride exposure.
- Findings revealed variable expression patterns of BmGSTs across tissues, highlighting their distinct responses to insecticides and fluoride, which may aid in creating pesticide-resistant silkworms.
Article Abstract
Glutathione S-transferases (GSTs) are a multifunctional super gene family, some of which play an important role in insecticide resistance. In this research, we used a real-time quantitative RT-PCR method, and a novel strategy, to measure the transcriptional level per gene copy using an exogenous RNA reference and DNA reference. The transcription levels of six BmGST genes in different tissues of fifth instar Bombyx mori larvae and their responses to insecticide and fluoride were investigated. The results show different levels and patterns of expression of the different BmGSTs in the various tissues observed. The BmGSTs can be induced by insecticide and fluoride, but their responses to each are different. The results of this research are helpful in studying the tissue-specific expression of BmGSTs in Bombyx mori, and in developing new pesticide resistant silkworm varieties.
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| http://dx.doi.org/10.1007/s11033-010-0626-9 | DOI Listing |
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