Purpose: To test the hypothesis that Cdk5/p35 plays important roles during trigeminal neuropathic pain.
Methods: Trigeminal neuralgia rat model was established with a chronic constriction injury (CCI) of the infraorbital branch of the trigeminal nerve (ION). The change of Cdk5 activity, expression of Cdk5 and p35 in Vc after CCI-ION were studied by Western blot, immunoprecipitation and Kinase assay. Statistical analysis was performed using SPSS13.0 software package.
Results: Western blot showed CCI-ION induced a time-dependent upregulation of p35 primarily within the ipsilateral superficial laminae of Vc (day 1:1.23±0.15, day 3:1.36±0.12, day 7:1.62±0.17, day 14:1.83±0.16). In contrast, the expression of Cdk5 was constant during day 1-14 in Vc after CCI-ION. Cdk5 activity on day 14 in Vc after CCI-ION (115.5 Kcpm) was 6 times as that on day 1 in Vc after CCI-ION (19.0 Kcpm). The difference was significant (P<0.01).
Conclusions: The results suggest that cdk5/p35 may plays important roles on synaptic reorganization of Vc after CCI-ION, expression of p35 may be a novel regulatory mechanism to control cdk5 activity in Vc after CCI-ION.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Blocking the p38 MAPK Signaling Pathway in the Rat Hippocampus Alleviates the Depressive-like Behavior Induced by Spinal Cord Injury.
ACS Chem Neurosci
January 2025
Jiangxi Key Laboratory of Neurological Diseases, Department of Neurosurgery, the first Affiliated Hospital, Jiangxi Medical College, Nanchang University, No. 17 Yongwaizheng Street, Nanchang, Jiangxi 330006, China.
Patients with spinal cord injury (SCI) may develop depression, which can affect their rehabilitation. However, the underlying mechanism of depression in SCI patients remains unclear. Previous studies have revealed increased p38 MAPK phosphorylation in the rat hippocampus after SCI, accompanied by depression-like behaviors.
View Article and Find Full Text PDFSIRT4 Protects Retina Against Excitotoxic Injury by Promoting OPA1-Mediated Müller Glial Cell Mitochondrial Fusion and GLAST Expression.
Invest Ophthalmol Vis Sci
January 2025
Affiliated Eye Hospital of Nanchang University, Jiangxi Research Institute of Ophthalmology and Visual Science, Jiangxi Provincial Key Laboratory for Ophthalmology, Jiangxi Clinical Research Center for Ophthalmic Disease, Nanchang, China.
Purpose: This study aimed to investigate the role of SIRT4 in retinal protection, specifically its ability to mitigate excitotoxic damage to Müller glial cells through the regulation of mitochondrial dynamics and glutamate transporters (GLASTs).
Methods: A model of retinal excitatory neurotoxicity was established in mice. Proteins related to mitochondrial dynamics, GLAST, and SIRT4 were analyzed on days 0, 1, 3, and 5 following toxic injury.
Sexual dimorphism in right ventricular adaptation to pressure overload involves differential angiogenic response.
Am J Physiol Heart Circ Physiol
January 2025
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS.
This study investigated the sexual dimorphism in right ventricle (RV) remodeling in right heart failure susceptible Fischer CDF rats using the pulmonary artery banding (PAB) model. Echocardiography and hemodynamic measurements were performed in adult male and female Fischer CDF rats at 1- or 2-weeks post-PAB. RV systolic pressure and RV hypertrophy were significantly elevated in PAB rats compared to sham control at 1- and 2-weeks post-PAB; however, no differences were observed between male and female rats.
View Article and Find Full Text PDFThe Impact of Levothyroxine and Testosterone Administration on Bladder Contractility in the Rat Model of Propylthiouracil-Induced Hypothyroidism.
Urol Res Pract
January 2025
Department of Pharmacology, Ankara University, Faculty of Pharmacy, Ankara, Türkiye.
Objective: To investigate the effects of testosterone (T) treatment, with or without levothyroxine, the most widely used and least effective medication for managing hypothyroidism, on the functional and histological changes in propylthiouracil (PTU)- induced hypothyroid rat bladders.
Methods: Male rats (n=35) were split into control, hypothyroid, hypothyroid rats treated with levothyroxine (20 µg/kg/day, oral, 2-weeks), hypothyroid rats treated with Sustanon (10 mg/kg,iIM, once/week, 2-weeks), and hypothyroid rats treated with combined treatment groups. Hypothyroidism was induced by PTU (0.
The Effect of St. John's Wort Oil on Urethral Stricture: An Experimental Study in Rat Model.
Urol Res Pract
January 2025
Department of Urology, Adıyaman University, Faculty of Medicine, Adıyaman, Türkiye.
Objective: The aim was to evaluate the effect of yellow and red centaury St. John's Wort (St. JW) oil on inflammation and urethral fibrosis development in a rat urethral trauma model.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!