The present study was aimed at investigating effects of nickel (NiCl(2)) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000μmol/L NiCl(2) or without NiCl(2) using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of ≥250μmol/L NiCl(2). A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000μmol/L NiCl(2). After addition of ≥250μmol/L NiCl(2) higher incidence of euchromatin was observed. Lipid droplets and vacuoles in cytoplasm were increased after addition of ≥125μmol/L NiCl(2). NiCl(2) induced decrease in numbers of mitochondria and smooth endoplasmic reticulum after treatment with ≥500μmol/L NiCl(2). Our findings suggest a negative effect of NiCl(2) on steroidogenesis, viability, apoptosis and ultrastructure of mouse Leydig cells.
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http://dx.doi.org/10.1016/j.jtemb.2010.11.003 | DOI Listing |
Adv Sci (Weinh)
January 2025
Department of Food Science and Nutrition, Pukyong National University, Busan, 48513, Republic of Korea.
The physical abrasion of plastics from simple everyday entered the food chain, with associated risks recently emphasized. Although many studies have reported the adverse effects of microplastics (MPs) on human, the reproductive implications of continuous exposure to physically abraded polyethylene terephthalate (PET)-MPs remain unexplored. Ingestion of physically abraded PET-MPs (size range: 50-100 µm) in mice from 5 to 34 weeks of age at an annual intake relevant dose of MPs (5 mg week) significantly impaired male reproductive function.
View Article and Find Full Text PDFElife
January 2025
Laboratory of Bone and Adipose Biology, Shanxi Medical University, Taiyuan, China.
The FSH-FSHR pathway has been considered an essential regulator in reproductive development and fertility. But there has been emerging evidence of FSHR expression in extragonadal organs. This poses new questions and long-term debates regarding the physiological role of the FSH-FSHR, and underscores the need for reliable, in vivo analysis of FSHR expression in animal models.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi 030800, China. Electronic address:
Sulfur dioxide (SO) is a ubiquitous environmental pollutant that has been shown to be toxic to the male reproductive system, but the underlying mechanism remains unclear. Therefore, the SO-treated mice and primary Leydig cell models were established to investigate the effects of SO on the production of testosterone and its specific mechanism. The results demonstrated that SO activated the ERK1/2 signaling pathway, leading to increased key proteins expression of testosterone biosynthesis and elevated testosterone levels.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
Reproductive Medicine Center, Henan Provincial People's Hospital, Zhengzhou, China; Reproductive Medicine Center, Zhengzhou University People's Hospital, Zhengzhou, China; Henan Joint International Research Laboratory of Reproductive Bioengineering, Zhengzhou, China. Electronic address:
4-Cyano-4'-ethoxybiphenyl (EBCN) is a representative cyano liquid crystal monomer (LCM). While prior studies have documented the widespread occurrence of LCMs in diverse environmental and biological samples, research on their reproductive effects in vivo remains limited. This study employed 35-day and 70-day exposure models in mice to assess the short-term and long-term effects of environmentally relevant concentrations of EBCN on testicular health.
View Article and Find Full Text PDFJ Ethnopharmacol
December 2024
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, China; Department of Urology, Shandong Provincial Hospital, Associated with Shandong University, Jinan, 250021, China; College of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250199, China; Key Laboratory of Urinary Diseases in Universities of Shandong, Shandong First Medical University, Jinan, 250021, China. Electronic address:
Ethnopharmacological Relevance: Late-onset hypogonadism (LOH) is a common age-related condition in men, characterized by a decline in serum testosterone and a range of associated signs and symptoms, and is classified as impotence in traditional medicine. Saffron is the dried stigma of Crocus sativus L., which is used in traditional medicine as an aphrodisiac.
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