AI Article Synopsis
- MicroRNAs (miRNAs) are crucial in regulating synaptic structure and function, specifically the miR-310-313 cluster, which is essential for proper neurotransmission at the Drosophila larval neuromuscular junction.
- Loss of the miR-310-313 cluster results in enhanced neurotransmitter release, but this can be reversed by reintroducing the miRNA in presynaptic neurons.
- The miR-310-313 cluster targets the Khc-73 protein, and its repression is necessary for maintaining normal synaptic function and homeostasis, as evidenced by increased levels of active zone proteins in cluster mutants.
Article Abstract
Emerging data implicate microRNAs (miRNAs) in the regulation of synaptic structure and function, but we know little about their role in the regulation of neurotransmission in presynaptic neurons. Here, we demonstrate that the miR-310-313 cluster is required for normal synaptic transmission at the Drosophila larval neuromuscular junction. Loss of miR-310-313 cluster leads to a significant enhancement of neurotransmitter release, which can be rescued with temporally restricted expression of mir-310-313 in larval presynaptic neurons. Kinesin family member, Khc-73 is a functional target for miR-310-313 as its expression is increased in mir-310-313 mutants and reducing it restores normal synaptic function. Cluster mutants show an increase in the active zone protein Bruchpilot accompanied by an increase in electron dense T bars. Finally, we show that repression of Khc-73 by miR-310-313 cluster influences the establishment of normal synaptic homeostasis. Our findings establish a role for miRNAs in the regulation of neurotransmitter release.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034365 | PMC |
| http://dx.doi.org/10.1016/j.neuron.2010.11.016 | DOI Listing |
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