Background: In light of increasing attention focused on patient safety and the need to reduce laboratory mistakes, it is crucial for clinical laboratories to collect statics on error rates over the whole testing cycle, particularly on the pre-analytical phase.
Methods: Memorial Hospital has a laboratory service providing stat and routine tests. From January 2005 to December 2007, a total of 766,095 specimens were received in our laboratory. The frequencies and types of pre-analytical errors and the potential targets for quality improvement strategies were evaluated.
Results: The relative frequencies of pre-analytical errors were ordered in a gradually decreasing trend through years; 0.50% in 2005; 0.25% in 2006 and 0.07% in 2007 (p < 0.0001; between 2005 and 2007). The most common mistake was incorrect/missing identification (42 %).
Conclusions: We believe that the use of standardized routines and regular staff training, combined with an exchange of the existing paper-based referral management system with an electronic system, can increase the safety in the pre-analytical processes.
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Pathologica
October 2024
Division of Experimental Oncology, Istituto Europeo di Oncologia, IRCCS, Milano.
Objective: Prostate cancer (PCa) is the most common cause of cancer-related deaths in men worldwide. BRCA1/2 genes are reported altered in approximately 1% and 8% of PCa cases, respectively. To date, formalin-fixed paraffin-embedded (FFPE) tissues have a consolidate use in the clinical practice, but with a significant drawback related to DNA/RNA degradation during the pre-analytical process.
View Article and Find Full Text PDFRev Med Inst Mex Seguro Soc
February 2024
Instituto de Seguridad Social para los Trabajadores del Estado, Hospital Fernando Quiroz Gutiérrez, Servicio de Cardiología. Ciudad de México, México.
Background: Errors in the pre-analytical phase of the microbiology laboratory reduce patient safety and generate additional expenses. In Mexico we do not have systems for evaluation and monitoring of the pre-analytical phase; quality indicators are proposed for continuous improvement.
Objective: Identify the main reasons for rejection of respiratory samples in the microbiology laboratory and evaluate the usefulness of preanalytical quality indicators.
J Mol Diagn
January 2025
The SARS-CoV-2 Whole Genome Sequencing Working Group of the Association for Molecular Pathology, Rockville, Maryland; Diagnostic Medicine Institute, Geisinger, Danville, Pennsylvania; Geisinger Commonwealth School of Medicine, Scranton, Pennsylvania.
Next-generation sequencing (NGS) has applications in research, epidemiology, oncology, and infectious disease diagnostics. Wide variability exists in NGS wet laboratory techniques and dry laboratory analytical considerations. Thus, many questions remain unanswered when NGS methods are implemented in laboratories for infectious disease testing.
View Article and Find Full Text PDFCureus
September 2024
Department of Pathology, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Introduction Errors occur in the laboratory at any level of the testing process. Recognizing these errors may cause patient distress by delaying diagnosis and management until results are released. This study aims to assess preanalytical errors in the laboratory and suggests methods to prevent them to improve accuracy and efficiency in the hematology department of a tertiary care hospital.
View Article and Find Full Text PDFSci Rep
August 2024
Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
Formalin-fixed paraffin-embedded (FFPE) tissue represents a valuable source for translational cancer research. However, the widespread application of various downstream methods remains challenging. Here, we aimed to assess the feasibility of a genomic and gene expression analysis workflow using FFPE breast cancer (BC) tissue.
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