AI Article Synopsis

  • A small percentage of Waldenstrom macroglobulinemia (WM) cases show biclonality, where two different VDJ gene segments are rearranged.
  • Researchers explored a WM patient with two distinct clones (V(H)3 and V(H)4) and found that only the V(H)4 clone expanded in a 3-D culture of WM bone marrow.
  • The V(H)4 clone exhibited characteristics of cancer stem cells and might be more clinically relevant than the V(H)3 clone, highlighting potential for better monitoring and treatment of WM.

Article Abstract

A small percentage of cases of Waldenstrom macroglobulinemia (WM) present with biclonality, defined here as the rearrangement of two distinct VDJ gene segments. Here we investigated the expansion of two clones from a patient with WM expressing molecularly detectable clonotypic gene rearrangements, one V(H)3 and one V(H)4. Biclonality was determined in blood and bone marrow mononuclear cells using real-time quantitative PCR (RQ-PCR). V(H)4 expressing cells but not V(H)3 expressing cells underwent clonal expansion in 3-D culture of reconstructed WM bone marrow. After 3-D culture, secondary culture in a colony forming unit assay, and RQ-PCR, only the V(H)4 clone was shown to harbor a subpopulation with characteristics of cancer stem cells, including proliferative quiescence, self-regeneration, and the ability to generate clonotypic progeny, suggesting that the V(H)4, but not the V(H)3, clone is clinically significant. Enrichment of potential WM stem cells in 3-D cultures holds promise for monitoring their response to treatment and for testing new therapies.

Download full-text PDF

Source
http://dx.doi.org/10.3109/10428194.2010.535183DOI Listing

Publication Analysis

Top Keywords

stem cells
12
waldenstrom macroglobulinemia
8
cancer stem
8
bone marrow
8
rq-pcr vh4
8
expressing cells
8
3-d culture
8
cells
6
patient biclonal
4
biclonal waldenstrom
4

Similar Publications

Various mature tissue-resident cells exhibit progenitor characteristics following injury. However, the existence of endogenous stem cells with multiple lineage potentials in the adult spinal cord remains a compelling area of research. In this study, we present a cross-species investigation that extends from development to injury.

View Article and Find Full Text PDF

Artificially induced haploidy is lethal in vertebrates, although it is useful for genetic screening and genome editing due to its single set of genomes. Haploid embryonic stem (ES) cell lines in mammals contribute to genetic studies and the production of gametes derived from haploid ES cells. In fish breeding, doubled haploids (DHs) induced by artificially induced gynogenesis are used to generate isogenic gametes for cloning purposes.

View Article and Find Full Text PDF

Engineering the Ratios of Nanoparticles Dispersed in Triphasic Nanocomposites for Biomedical Applications.

ACS Appl Mater Interfaces

January 2025

Department of Bioengineering, University of California, Riverside, 900 University Avenue, Riverside, California 92521, United States.

Polymer/ceramic nanocomposites integrated the advantages of both polymers and ceramics for a wide range of biomedical applications, such as bone tissue repair. Here, we reported triphasic poly(lactic--glycolic acid) (PLGA, LA/GA = 90:10) nanocomposites with improved dispersion of hydroxyapatite (HA) and magnesium oxide (MgO) nanoparticles using a process that integrated the benefits of ultrasonic energy and dual asymmetric centrifugal mixing. We characterized the microstructure and composition of the nanocomposites and evaluated the effects of the HA/MgO ratios on degradation behavior and cell-material interactions.

View Article and Find Full Text PDF

Osteoporosis, affecting the entire skeletal system, can cause bone mass to diminish, thereby reducing bone strength and elevating fracture risk. Fracture nonunion and bone defects are common in patients with fractures, and pain and loss of function may cause serious distress. The search for a new therapeutic strategy is essential because of the limited therapeutic options available.

View Article and Find Full Text PDF

Regenerative Potential of Neural Stem/Progenitor Cells for Bone Repair.

Tissue Eng Part B Rev

January 2025

Research Unit in Mineralized Tissue Reconstruction and Faculty of Dentistry, Thammasat University, Pathum Thani, Thailand.

The increasing number of elderly people across the globe has led to a rise in osteoporosis and bone fractures, significantly impacting the quality of life and posing substantial health and economic burdens. Despite the development of tissue-engineered bone constructs and stem cell-based therapies to address these challenges, their efficacy is compromised by inadequate vascularization and innervation during bone repair. Innervation plays a pivotal role in tissue regeneration, including bone repair, and various techniques have been developed to fabricate innervated bone scaffolds for clinical use.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!