AI Article Synopsis
- The study evaluated over 200 antibodies targeting 57 histone modifications in fruit flies, worms, and human cells.
- While many antibodies worked effectively, over 25% did not pass specificity tests, and among the specific ones, more than 20% failed in further experimental applications.
- The authors recommend careful testing of these antibodies before use and created a website for sharing new test results.
Article Abstract
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017233 | PMC |
| http://dx.doi.org/10.1038/nsmb.1972 | DOI Listing |
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