The rational design of artificial enzymes, either by applying physico-chemical intuition of protein structure and function or with the aid of computational methods, is a promising area of research with the potential to tremendously impact medicine, industrial chemistry and energy production. Designed proteins also provide a powerful platform for dissecting enzyme mechanisms of natural systems. Artificial enzymes have come a long way from simple α-helical peptide catalysts to proteins that facilitate multistep chemical reactions designed by state-of-the-art computational methods. Looking forward, we examine strategies employed by natural enzymes that could be used to improve the speed and selectivity of artificial catalysts.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3443871 | PMC |
| http://dx.doi.org/10.1038/nchem.473 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of aluminum on metabolism of reactive oxygen species and reactive nitrogen species in root tips of different Eucalyptus species.
BMC Plant Biol
January 2025
Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning, 530004, China.
On acidified soil, the growth of Eucalyptus is seriously restricted by aluminum (Al) stress. Therefore, breeding Eucalyptus species with excellent Al tolerance, developing the genetic potential of species, and improving tolerance to Al stress are important for the sustainable development of artificial Eucalyptus forests. By observing the occurrence and distribution of the main reactive oxygen species (ROS) and reactive nitrogen species (RNS) in root tips of Eucalyptus seedlings under Al stress, this study analyzed change in the growth and physiological indexes of Eucalyptus seedlings under Al stress.
View Article and Find Full Text PDFConstruction of isopentenol utilization pathway and artificial multifunctional enzyme for miltiradiene synthesis in Saccharomyces cerevisiae.
Bioresour Technol
January 2025
School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China; Frontiers Science Center for Synthetic Biology, Tianjin University, Tianjin, PR China; Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, PR China. Electronic address:
Miltiradiene serves as a pivotal precursor for the synthesis of numerous abietane-type diterpenes with important pharmacological activities. The endogenous mevalonate (MVA) pathway is tightly regulated in Saccharomyces cerevisiae, which limits the availability of precursors for the heterologous production of miltiradiene. In this study, the orthogonal isopentenol utilization pathway (IUP) was constructed and investigated for its adaptability with mitochondria and peroxisomes in S.
View Article and Find Full Text PDFWarfarin-induced gastrointestinal bleeding with acute liver failure: A case report.
Medicine (Baltimore)
November 2024
Department of Pharmacy, The People's Hospital of Hezhou, Hezhou, China.
Rationale: Warfarin is the most commonly used drug in patients with mechanical valve replacement. Acute liver damage after warfarin is rare but potentially harmful. We present a case of warfarin-induced gastrointestinal bleeding with liver injury, pharmacy monitoring, and its therapy.
View Article and Find Full Text PDFCharacterization and design of dipeptide media formulation for scalable therapeutic production.
Appl Microbiol Biotechnol
January 2025
School of Chemical Engineering, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-GuGyeonggi-Do 16419, Suwon-Si, South Korea.
Process intensification and simplification in biopharmaceutical manufacturing have driven the exploration of advanced feeding strategies to improve culture performance and process consistency. Conventional media design strategies, however, are often constrained by the stability and solubility challenges of amino acids, particularly in large-scale applications. As a result, dipeptides have emerged as promising alternatives.
View Article and Find Full Text PDFAge, creatinine, and ejection fraction score is a risk factor for acute kidney injury after surgical aortic valve replacement.
Ren Fail
December 2025
Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China.
Background: The incidence of acute kidney injury (AKI) increases after surgical aortic valve replacement (SAVR). This study aimed to characterize the risk factors of AKI after SAVR.
Methods And Results: We conducted a retrospective registry study based on data from 299 consecutive patients undergoing SAVR.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!