The effect of L-lysine HCl concentrations, pH, temperature and cultivation conditions on the synthesis of intracellular lysine decarboxylase (LDC) by Vibrio sp. was studied. The highest LDC activity was observed in a 4-hour culture grown at 30 degrees on a nutrient medium, pH 5.15 containing 0.9% L-lysine HCl as an inducer. Under these conditions the LDC activity of the bacterium was 8.43 U/mg dry cells.
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Bioengineering (Basel)
December 2024
CJ BIO Research Institute, CJ CheilJedang Corp., Suwon-si 16495, Gyeonggi-do, Republic of Korea.
The amino acid industry generates significant amounts of electrolyte residues, such as ammonium sulfate, acetic acid, and phosphoric acid, which cause challenges to sustainability. This short article investigates the feasibility of implementing a plant-scale circular economy through the recycling and biological reuse of these electrolyte residues. Scenario analyses of L-lysine (LYS) HCl, L-methionine (MET), and L-cysteine (CYS) HCl production highlight the environmental and economic benefits of the plant-scale circular economy.
View Article and Find Full Text PDFEur J Pharm Sci
February 2025
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:
Castrate-resistant prostate cancer (CRPC) is one of the most difficult cancers in men and is characterized by a poor prognosis and a high risk of metastasis. The overexpression of the disruptor of telomeric silencing 1-like (DOT1L), which is a specific methyltransferase for histone H3 at lysine residue 79 (H3K79), has been related to poor outcomes in patients with CRPC. Therefore, targeting DOT1L is considered a potential therapeutic approach to overcome the significant medical challenges of CRPC.
View Article and Find Full Text PDFAntib Ther
October 2024
Product Development, Catalent Pharma Solutions, 14 School House Rd, Somerset, NJ 08873, United States.
Background: Formulation screening is essential to experimentally balance stability and viscosity in high-concentration mAb formulations. We developed a high-throughput approach with automated sample preparation and analytical workflows to enable the integrated assessment of excipient compatibility and viscosity of mAb formulations.
Methods: Ninety-six formulations of a trastuzumab biosimilar were screened by combining 8 types of excipient modifiers with 4 types of buffers across a pH range of 4.
Vet Med Sci
November 2024
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Acta Pharm
September 2024
University of Ljubljana Faculty of Pharmacy, Department of Pharmaceutical Chemistry, SI-1000 Ljubljana Slovenia.
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