AI Article Synopsis
- Different methods used in this study reveal that serum proteins and sonication can significantly change the properties and toxicity levels of copper (Cu) nanoparticles.
- The study employed various techniques to analyze particle characteristics and toxic effects on human lung cells, finding that sonicated nanoparticles led to lower cell viability and higher copper release.
- While serum in the cell culture reduced agglomeration and increased copper release, it did not notably change the toxicity observed, with very few cells retaining intracellular copper nanoparticles due to rapid dissolution.
Article Abstract
Abstract Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.
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| http://dx.doi.org/10.3109/17435390.2010.536268 | DOI Listing |
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