AI Article Synopsis
- The study measured changes in lysosomal pH in human embryonic fibroblasts and Fabry's disease cells under sucrose loading over 6 to 120 hours using a dye called neutral red.
- It was found that long-term accumulation of non-hydrolysable compounds in lysosomes led to an average pH increase of 0.4 units, with the highest pH values (7.0-7.2) occurring in larger lysosomes of diseased cells.
- The increase in intralysosomal pH might hinder the function of acidic hydrolases, which are crucial for breaking down materials inside lysosomes.
Article Abstract
The changes in intralysosomal pH were measured in the stationary phase of normal human embryonic fibroblast growth under sucrose loading over a period of 6 to 120 hours and in cells with a typical lysosomal storage pathology, Fabry's disease, using a vital indicator dye, neutral red. It was shown that long-term hypertrophy of the lysosomal compartment during intracellular accumulation of non-hydrolysable compounds is concomitant with a pH increase, on the average, by 0.4 units. The highest values of pH (7.0-7.2) were seen in large-sized heterogeneic lysosomes of pathological cells. It is suggested that an increase in intralysosomal pH during accumulation of non-hydrolysable compounds leads to deterioration of conditions that are favourable for the acidic hydrolase function.
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