This paper describes a high yielding coupled enzymatic reaction using Bacillus halodurans purine nucleoside phosphorylase (PNP) and E. coli uridine phosphorylase (UP) for synthesis of 5-methyluridine (5-MU) by transglycosylation. Key parameters such as reaction temperature, pH, reactant loading, reactor configuration and enzyme loading were investigated. A guanosine conversion of 95% and a 5-MU yield of 85% were achieved at 1l scale, with a productivity of 10 g l⁻¹ h⁻¹.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiotec.2010.11.013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A flexible and scalable synthesis of 4'-thionucleosides.
Chem Sci
December 2024
Department of Chemistry, Simon Fraser University Burnaby British Columbia V5A 1S6 Canada
4'-Thionucleosides (thNAs) are synthetic nucleoside analogues that have attracted attention as leads for drug discovery in oncology and virology. Here we report a thNA synthesis that relies on a scalable α-fluorination and aldol reaction of α-heteroaryl acetaldehydes followed by a streamlined process involving carbonyl reduction, mesylate formation and a double displacement reaction using NaSH. We demonstrate the multigram preparation of 4'-thio-5-methyluridine and highlight the production of purine and pyrimidine thNAs as well as C2'-modified thNAs.
View Article and Find Full Text PDFDeep-m5U: a deep learning-based approach for RNA 5-methyluridine modification prediction using optimized feature integration.
BMC Bioinformatics
November 2024
Department of Computer Science, Khurasan University, Jalalabad, Afghanistan.
Background: RNA 5-methyluridine (m5U) modifications play a crucial role in biological processes, making their accurate identification a key focus in computational biology. This paper introduces Deep-m5U, a robust predictor designed to enhance the prediction of m5U modifications. The proposed method, named Deep-m5U, utilizes a hybrid pseudo-K-tuple nucleotide composition (PseKNC) for sequence formulation, a Shapley Additive exPlanations (SHAP) algorithm for discriminant feature selection, and a deep neural network (DNN) as the classifier.
View Article and Find Full Text PDFDecoding Substrate Selectivity of an Archaeal RlmCD-like Methyltransferase Through Its Salient Traits.
Biochemistry
October 2024
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
5-Methyluridine (mU) rRNA modifications frequently occur at U747 and U1939 ( numbering) in domains II and IV of the 23S rRNA in Gram-negative bacteria, with the help of -adenosyl-l-methionine (SAM)-dependent rRNA methyltransferases (MTases), RlmC and RlmD, respectively. In contrast, Gram-positive bacteria utilize a single SAM-dependent rRNA MTase, RlmCD, to modify both corresponding sites. Notably, certain archaea, specifically within the group, have been found to possess two genes encoding SAM-dependent archaeal (tRNA and rRNA) mU (ArmU) MTases.
View Article and Find Full Text PDFModulation of liver metabolism and gut microbiota by Alhagi-honey alleviated heat stress-induced liver damage.
Stress Biol
September 2024
College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, China.
Alhagi-honey (AH) is a well-established traditional ethnic medicine with advantageous effects against diarrhea and headaches. We aimed to explore the preventive effect of AH on liver damage induced by heat stress (HS) and its underlying mechanism. HS models were established by thermostat, and mice were treated at 39 ℃ for 10 h, lasting for 7 days.
View Article and Find Full Text PDFCombining Nanopore direct RNA sequencing with genetics and mass spectrometry for analysis of T-loop base modifications across 42 yeast tRNA isoacceptors.
Nucleic Acids Res
October 2024
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Transfer RNAs (tRNAs) contain dozens of chemical modifications. These modifications are critical for maintaining tRNA tertiary structure and optimizing protein synthesis. Here we advance the use of Nanopore direct RNA-sequencing (DRS) to investigate the synergy between modifications that are known to stabilize tRNA structure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!