Determination of enzymatic activities and metabolites in microliter sample size.

Anal Biochem

Departamento de Bioquímica, Facultad de Medicina, Instituto de Investigaciones Biomédicas "Alberto Sols" CSIC-UAM, 28029 Madrid, Spain.

Published: March 2011

A new generation of spectrophotometers able to measure a wide range of absorbance in microliter aliquots is currently used for the determination of DNA, RNA, and proteins. The object of this article is to show that these instruments could be easily adapted for routine evaluation of enzymes and metabolites in 1-2-microl volumes of biological samples.

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http://dx.doi.org/10.1016/j.ab.2010.11.036DOI Listing

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